CD4^+ CD25^+ CD127^(low/-)Treg在慢性阻塞性肺疾病中的表达及意义

Clinical significance of CD4^+ CD25^+ CD127^( low/-) regulate T cells in patients with chronic obstructive pulmonary disease

目的通过检测COPD患者外周血中Treg细胞的表达水平,探讨COPD患者体内免疫功能的状态及Treg细胞在疾病进展中的临床意义。方法选择AECOPD和COPD稳定期患者各30例,20例健康体检者作为正常对照组,采用流式细胞仪进行检测外周血中CD4+CD25+Treg细胞、CD4+CD25+CD127(low/-)Treg细胞占CD4+T淋巴细胞比例。SPSS 17.0软件进行统计学分析处理。结果 AECOPD组和COPD组外周血中CD4+CD25+Treg细胞比例分别为(9.52±0.95)%和(9.72±0.97)%均低于对照组(10.22±1.10)%,差异无统计学意义(F=2.48,P=0.093)。3组间CD4+CD25+CD127(low/-)Treg细胞比例差异有统计学意义(F=13.57,P=0.0),AECOPD组(4.50±0.83)%显著低于COPD稳定期(5.22±0.64)%和对照组(5.61±0.67)%,而COPD稳定期较对照组降低,差异无统计学意义(P>0.05)。结论 COPD患者外周血中CD4+CD25+CD127(low/-)Treg细胞降低,存在免疫内环境的失衡,Treg细胞可能参与了COPD的发病和急性加重过程。

Objective To investigate the status of immune function, and explore the frequency and significance of CD4^＋ CD25^＋ CD127^（low/-） regulate T cells（Treg） in peripheral blood of patients with chronic obstructive pulmonary disease （COPD）. Methods CD4^＋ CD25^＋ Treg and CD4 ^＋ CD25^ ＋ CD127^（low/-） Treg cells in the peripheral blood were detect by flow cytometry in 30 patients with acute chronic obstructive pulmonary disease（AECOPD） ,30 patients with COPD at sta- ble stage and 20 normal control. The statistical analysis was conducted by SPSS software. Results There were no statisti- cal significance in percentage CD4^＋ CD25^＋ Treg cells among AECOPD group （ 9.52 _＋ 0.95 ） % , COPD group （ 9.72 ± 0.97 ） % and normal control group（ 10.22 ± 1.10） % , F = 2.48,P = 0. 093. The percentages of CD4^＋ CD25^＋ CD127^（low/-） regulate T cell in AECOPD group （ 4.50 ± 0.83 % ） were significantly lower than that in COPD group （ 5.22 ± 0.64 ） % at stable stage and normal control（5.61 ± 0.67 ）%, P 〈 0.05 ;there was no significant between COPD group and control group（ P 〉 0.05 ）. Conclusion CD4^＋ CD25^＋ CD127^（low/-） regulate T cells in COPD patients were diminished, Treg cells may be involved in the pathogenesis of COPD and acute exacerbation.