摘要
目的分析乙肝病毒标记物阳性表型(Pres1、HBcAb-IgM、HBsAg、HBsAb、HBeAg、HBeAb、HBcAb-IgG中两项或两项以上阳性)血清标本中HBV-DNA水平,为乙型病毒性肝炎的诊断、病情发展及预后评估提供理论依据。方法对以ELISA法检测Pres1、HBcAb-IgM、HBsAg、HBsAb、HBeAg、HBeAb、HBcAb-IgG项目中两项或两项以上同时阳性的652例标本用荧光定量PCR法检测HBV-DNA水平,并对检测结果进行统计分析。结果非活动期标记物表型组可出现高水平HBV-DNA;正常对照组和非活动期标记物表型组血清HBV-DNA平均水平均明显低于活动期标记物表型组(P<0.01);高水平HBV-DNA标本中不一定会同时出现Pres1、HBeAg、HBcAb-IgM等活动期标记物。结论活动期乙肝病毒标记物检测阴性不能排除患者体内是否存在乙肝病毒复制,Pres1、HBeAg、HBcAb-IgM联合检测可以提高病毒感染阳性检出率,同时可以评估疾病的进展情况。
Objective To study the results of HBV-DNA in serum which hepatitis B virus markers were positive. Meth- otis HBV-DNA in 652 cases of serum were measured by polymerase chain reaction （ PCR）, Presl, HBeAb-lgM, HbsAg, HbsAb, HbeAg, HbeAb, HBcAb-IgG were measured by enzyme linked immunosorbent assay （ELISA） , control group Was composed of 50 healthy people. Results The levels of HBV-DNA of patient group were higher than that of control group （P 〈0. 01 ）. Among the 778 samples, 11 kinds of makes group appeared. Sometimes, the level of HBV-DNA in serum which Presl, HbeAg, HBcAb-IgM are all negative was higher than that of control group（ P 〈 0.01 ）. Conclusion It is im- portant to measure HBV-DNA, Presl, HbeAg, HBcAb-IgM in serum the same time for the treatment and diagnosis to hepa- titis B.
出处
《中华全科医学》
2014年第6期977-978,共2页
Chinese Journal of General Practice
