金标免疫层析法检测儿童腹泻粪便标本轮状病毒及结果分析

Testing and results analysis of rotavirus in stool specimens of children with diarrhea

目的使用金标免疫层析法检测了解儿童腹泻粪便标本轮状病毒,并采用RT-PCR法对检测结果进行验证,判断检测结果的可靠性寻找其流行病学特征,为应用轮状病毒疫苗提供研究数据。方法对124例轮状病毒金标免疫层析法检测阳性的儿童腹泻患者粪便标本进行回顾性分析,对124例儿童腹泻患者粪便标本进行回顾性分析,采用金标免疫层析法检测轮状病毒呈阳性。分析标本的年龄分布与季节分布;采用RT-PCR法对标本的轮状病毒进行验证,并检测标本的星状病毒、诺如病毒和腺病毒感染情况。结果 124例患儿的平均年龄(1.2±0.3)岁,年龄为0岁者的例数最多,占41.13%(51/124);随着年龄的升高,标本数越来越少。124例标本中,12月、1月、11月、2月、10月的例数较多;轮状病毒导致的儿童腹泻多发于冬季和秋季,少发于夏季和春季。RTPCR法检出轮状病毒阳性者120例,金标免疫层析法与RT-PCR法检测结果的相符率为96.77%。124例腹泻患儿中合并星状病毒感染者20例,占16.13%;合并诺如病毒者9例,占7.26%;合并腺病毒者27例,占21.77%。结论金标免疫层析法操作简便,可快速准确实现对儿童腹泻粪便标本的轮状病毒检测。星状病毒、诺如病毒和腺病毒也是导致儿童腹泻的病原体。

Objective To compare the results of gold nanoparticle-based immunochromatographic assay and RT-PCR tes- ting rotavirus in stool specimens of children with diarrhea, and evaluate the reliability of gold nanoparticle-based immuno- chromatographic assay. Methods The gold nanopartiele-based immunochromatographic assay and RT-PCR were em- ployed to test rotavirus in stool specimens of 124 children with diarrhea, and the results were compared. The astrovirus, no- rovirus and adenovirus in specimens were also detected. Results The average age of 124 children was （ 1.2 ＋-0.3 ） years of age,most of them（51/124 accounting for 41.13% ） were less than 12 months,the number of specimens decreased with the age. The case number was more in December, January, November, February and October. In 124 positive specimens by gold nanoparticle-based immunochromatographic assay, 120 cases were with positive results by RT-PCR with a consistent rate of 96.77%. 20 cases were complicated with astrovirus infection, accounting for 16.13% ;9 cases with norovirus, ac- counting for 7.26% ;and 27 cases with adenovirus, accounting for 21.77%. Conclusion Gold nanoparticle-based immu- nochromatographic assay is easy to operate and can detect accurately and quickly rotavirus in stool specimens of children with diarrhea.