摘要
目的：研究平喘方对哮喘模型小鼠Rho/Rock信号通路表达的影响，探讨平喘方治疗哮喘的机理。方法采用随机数字表法将40只BALB/C小鼠随机分为空白对照组、哮喘模型组、地塞米松组和平喘方干预组各10只。给药干预4周后用Western blot法检测各组小鼠肺组织RhoA蛋白、RockI蛋白表达量，RT-PCR法检测小鼠肺组织RhoA mRNA、RockI mRNA表达量。结果哮喘模型组小鼠RhoA蛋白、RockI蛋白、RhoA mRNA、RockI mRNA表达量[分别为（1.05±0.20）、（1.06±0.08）、（6.60±1.09）、（6.53±1.84）]，均高于空白对照组[分别为（0.76±0.08）、（0.84±0.14）、（3.82±1.77）、（3.65±1.46）]，差异有统计学意义（P＜0.01）。地塞米松干预组小鼠RhoA蛋白、RockI蛋白、RhoA mRNA、RockI mRNA表达量[分别为（0.78±0.11）、（0.87±0.32）、（4.19±2.33）、（4.09±1.08）]，低于哮喘模型组（P＜0.01）。平喘方干预组[分别为（0.86±0.12）、（0.93±0.14）、（4.38±2.01）、（4.50±1.13）]低于哮喘模型组，差异有统计学意义（P＜0.05）；平喘方干预组与地塞米松干预组比较差异无统计学意义（P＞0.05）。结论平喘方可抑制哮喘小鼠Rho/Rock信号通路表达，效果与地塞米松相近。
Objective To study the effect of Pingchuan Formula（PCF）on Rho/Rock signaling pathway in the mice of asthmatic model, explore the mechanism of PCF on asthma. Methods Forty BALB/C mice were randomly divided into four groups：a control group, a model group,a dexamethasone group and a PCF group. After treated with medicine for 4 weeks, albumen expression of RhoA and RockI in lung tissues were detected by using Western blot, mRNA expressions of RhoA and RockI were detected by using Real-time PCR. Results The albumen and the mRNA expression of RhoA and RockI in the model group were（1.05± 0.20）,（1.06±0.08）,（6.60±1.09）, （6.53±1.84）, the control group were（0.76±0.08）, （0.84±0.14）, （3.82± 1.77）, （3.65±1.46）,there was a significant difference between two groups（P0.05）. Conclusion The expressions of Rho/Rock signaling pathway were controlled significantly by PCF, and the curative effect was similar to dexamethasone.
出处
《国际中医中药杂志》
2014年第5期435-437,共3页
International Journal of Traditional Chinese Medicine
基金
上海市卫生局科研项目（项目编号：2010Y127）
