氢化可的松致小鼠阳虚常用造模方法及指标比较

Comparison on model establishment methods and index in common of Yang deficiency mice induced by hydrocortisone

目的：比较和分析氢化可的松不同给药途径、剂量致小鼠阳虚模型相关指标的变化。方法以肌肉和腹腔注射两种途径给予小鼠高、低两种剂量（12.5 mg/kg、25 mg/kg氢化可的松制作阳虚模型，观察模型小鼠的症状、体质量、平均摄食量、应激指标以及性器官和免疫器官的脏器系数的改变。结果①腹腔注射给药途径，高剂量模型组的体质量（24.52±3.29）g、体温（35.24±0.32）℃明显低于对照组（31.10±6.11）g、（37.02±0.64）℃；低剂量模型组的平均摄食量（4.30±0.29）g/（只？d）明显低于对照组（7.38±0.53）g/（只？d）。低剂量模型组的包皮腺系数（0.10±0.02）、阴茎系数（0.15±0.03）、胸腺系数（0.12±0.03）均低于对照组（0.15±0.02、0.18±0.02、0.22±0.08、0.98±0.45）。②肌肉注射给药：高剂量模型组平均摄食量（5.92±2.01）g/（只？d），明显低于对照组（8.60±1.33）g/（只？d）；低剂量模型组体温（34.90±0.22）℃、低温游泳时间（34.00±22.41）s均低于对照组（36.43±0.91）℃、（67.17±21.93）s。两个模型组的胸腺系数（0.10±0.02）、（0.11±0.06）均低于对照组（0.17±0.00）、（0.25±0.03）。结论不同途径、不同剂量的氢化可的松给药致小鼠阳虚症状出现的时间、程度和相关敏感指标不同。

Objective To compare and analyze the index variation of Yang deficiency mice induced by hydrocortisone in different way of administration and dose. Methods The mice model of Yang deficiency were induced by low dose and high dose hydrocortisone（12.5 mg/kg, 25 mg/kg） in two ways of intramuscular and intraperitoneal injection. The symptoms, body weight, the average intake, the index of stress and the coefficient of sex organs and immune organs of animals were observed. Results In the way of intraperitoneal injection, the weight（24.52±3.29）g, body temperature（35.24±0.32）℃ of high dose of model group were significantly lower than that of control group（31.10±6.11）g,（37.02±0.64）℃. The average intake of low dose group（4.30 ± 0.29）g/（per？d） was lower than control group（7.38±0.53）g/（per？d）, and the coefficient of preputial glands（0.10±0.02）, penis（0.15±0.03）, thymus（0.12±0.03）were lower than that of control group. In the way of intramuscular injection, the average intake of high dose group（5.92±2.01）g/（per？d） was lower than control group（8.60±1.33）g/（per？d）. The body temperature（34.90±0.22）℃ and the time of swimming in low temperature （34.00±22.41）s of low dose model group were lower than that of control group（36.43±0.91）℃, （67.17±21.93）s, and the coefficient of thymus of two model groups（0.10±0.02）,（0.11±0.06）were lower than that of control group. Conclusion Various dose and model establishment methods of Yang deficiency mice have different the time of symptom appearing, the degree of symptom and sensitive index.