HPLC-ESI-MS快速筛选并鉴定补骨脂甲醇提取物中的抗氧化活性成分

Screening and identification antioxdants from methanol extract of psoralea corylifolia L.by HPLC-ESI-MS

采用HPLC-ESI-MS法建立了一种快速筛选并鉴定补骨脂甲醇提取物中抗氧化活性成分的方法,即利用二苯基三硝基苯肼(DPPH·)与补骨脂甲醇提取物中抗氧化活性成分反应前后色谱图中峰面积的变化来筛选出抗氧化活性物质,同时利用电喷雾质谱对这些活性组分进行结构鉴定.利用该方法从补骨脂甲醇提取物中筛选出了8个抗氧化活性组分,分别为新补骨脂异黄酮,补骨脂二氢黄酮,补骨脂异黄酮,补骨脂定,异补骨脂查尔酮,补骨脂二氢黄酮甲醚,corylifol和补骨脂酚.这种方法较传统的筛选方法省时省力,既不容易造成微量组分丢失,又不需要仪器改装工作,易于实现复杂体系抗氧化活性物质的高通量筛选.

HPLC-ESI-MS was used to screening and identify antioxdants from methanol extract of Pso- ralea corylifolia L.. Comparing the chromatograms of experiment and control group, the peak areas of antioxidants after the interaction with DPPH · had a decrease in the chromatogram while for those not responsible for DPPH · . These antioxidants were identified by ESI-MS. Eight antioxidants were screen out from the extract and were identified as Neobavaisoflavone, Bavachin, Corylin, Psoralidin, Isobavachalcone, Bavachinin, Corylifol, and Bakuchiol. This time-saving method can not only reduce the loss of trace components but also needn＇t additional instrument improvement. This method has the potential to be a high throughput tool for screening antioxidants from complicated systems such as herbal extracts.