芝麻显性细胞核雄性不育系内源激素、可溶性糖和淀粉含量变化

Variation of soluble sugar,starch and plant hormones contents in sesame dominant genic male sterile line during bud development

植物激素在花药发育过程中具有重要的调控作用。利用酶联免疫检测技术(ELISA),测定了芝麻显性细胞核雄性不育系叶片和花蕾中生长素(IAA)、脱落酸(ABA)、茉莉酸(JA)以及水杨酸(SA)含量的动态变化,分析了不同内源激素的平衡关系,同时比较了不育和可育株间的可溶性糖和淀粉含量差异。研究结果表明:1)在整个花药发育时期,不育株和可育株中的4种内源激素含量变化趋势明显不同,且含量差异明显;IAA含量的降低及ABA含量的提高可能与芝麻雄性不育的发生密切相关;2)不育株叶片中的IAA含量显著下降,JA和ABA显著上升;3)IAA/ABA、IAA/SA和IAA/JA在不育株和可育株间的变化趋势不一致,且差异很大,表明4种内源激素之间平衡关系受到破坏可能会影响到花蕾的正常生长发育;4)不育系花蕾中的可溶性糖含量、淀粉含量盈余,可能是导致雄性败育的原因之一。

Plant hormones play a key role in anther development. With the technology of enzyme- linked immunosorbent assay（ ELISA）,contents of four endogenous hormones（ IAA,ABA,JA and SA） in buds were evaluated at different developmental stages. The ratios of different hormones,as well as contents of soluble sugar and starch were compared among male sterile and fertile plants. The results indicated that： 1） during anther development,the variation trends and contents of four hormones were distinctive between male sterile and fertile plants,and the significant decrease of IAA and increase of ABA in male sterile plant could be connected with the occurrence of male sterility; 2） significant increase of JA and ABA and the decrease of IAA were observed in leaf in male sterile plants; 3） the variation trends and ratios of IAA / ABA,IAA / SA and IAA / JA were different among male sterile and fertile plants,indicating that the hormonal imbalance was also responsible for anther abortion; 4） the soluble sugar and starch contents in buds of male sterile plants were higher than that in fertile plants,which was at least partially responsible for male sterility.