间苯二酚-O,O′-二乙酸荧光猝灭法测定锑(Ⅲ)

Fluorescence quenching method for determination of antimony(Ⅲ) with m-phenylenedioxydiacetic acid

在OP-10乳化剂存在下，以NH3-NH4Cl缓冲溶液为介质，间苯二酚-O，O＇二乙酸（m—PA）为荧光试剂，建立了荧光猝灭法测定锑㈣的方法。考察了缓冲溶液酸度和用量、表面活性剂及其用量、m—PA溶液用量对测定体系的影响，并确定了最佳实验条件。结果表明，体系的最大发射波长λcm=609nm（最大激发波长λex=302nm），锑㈣含量在0．04～O．48μg／L范围内与荧光猝灭值△F呈良好的线性关系，方法检出限为0．0345μg／L。方法用于自来水、泉水和河水样品中锑㈣的测定，结果与原子吸收光谱法（AAS）一致，相对标准偏差（RSD，n=6）为3．2％～4．59／6。

A method was established for determination of antimony aid by fluorescence quenching meth- od in the presence of OP-10 emulsifier with NH4-NH4Cl buffer solution as medium and m-phenylened- ioxydiacetic acid （m-PA） as fluorescent reagent. The effects of acidity and dosage of buffer solution, the surfactant and its dosage as well as the dosage of m-PA solution on the test system were investiga- ted and accordingly the optimal experimental conditions were determined. The results showed that the system had a maximum emission wavelength λem = 609 nm （the maximum excitation wavelength ;λex = 302 nm）, antimony （Ⅲ） content in the range of 0.04-0.48 μg/L constituted a fine linear relationship with the fluorescence quenching value（AF） and the detection limit was 0. 034 5 μg/L. The method was applied to the determination of antimony （Ⅲ） in tap water, spring water and river water samples, the results were consistent with those obtained by atomic absorption spectrometry（AAS）, and the rel- ative standard deviations （RSD, n=6） were in the range of 3.2%-4.5%.