兔胸部爆炸伤后IL-6、IL-8及TNF-α的时间相关性表达

Time-related Expression of IL-6、IL-8 and TNF-α Following Explosive Injury to Rabbit's Chest

目的：探讨细胞因子白细胞介素6（interleukin-6，IL-6）、白细胞介素8（interleukin-8，IL-8）及肿瘤坏死因子α（tumor necrosis factor-α，TNF-α）在兔胸部爆炸伤后的含量变化与损伤时间的关系。方法建立家兔胸部爆炸伤的模型，应用酶联免疫吸附法（ELISA）测定不同损伤时间（0.5～12 h）血浆中IL-6、IL-8及TNF-α的表达水平。结果 IL-6在爆炸伤后3 h开始升高，6 h达峰值；IL-8在爆炸伤后1 h开始升高，6 h达峰值；TNF-α在爆炸伤后0.5 h开始升高，3 h达峰值。结论爆炸伤后IL-6、IL-8及TNF-α的蛋白表达水平呈时间相关性。

Objective To explore the relationship between the expression changes of cytokines, inter-leukin-6（IL-6）, interleukin-8（IL-8）, and tumor necrosis factor-α （TNF-α）, and the wound time following explosive injury to rabbit’s chest. Methods The rabbit’s model of explosive injury was established. The expression levels of IL-6、 IL-8 and TNF-α in the plasma were detected by ELISA method at different wound time （0.5-12 h）. Results The level of IL-6 increased at 3 h after wounding and reached peak at 6 h. The level of IL-8 increased at 1 h and reached peak at 6 h. The level of TNF-α increased at 0.5 h and reached peak at 3 h. Conclusion IL-6, IL-8 and TNF-α have a time-related expression after explo-sive injury.