LC-UV法同时测定南五味子和五味子中五味子醇甲、五味子酯甲的含量

Simultaneous Determination of Schizandrol A and Schisantherain A in Schisandra Sphenanthera Rehd. et Wils. and Schisandra Chinensis( Turcx. ) Baill. by RP- HPLC

目的:建立简单、快速、准确、通用的同时测定五味子醇甲、五味子酯甲含量的液相色谱-紫外检测(LC-UV)方法,为评价五味子品质提供参考。方法:色谱柱为Agilent TC-C18(2)(250 mm×4.6 mm,5μm),以甲醇-水为流动相,梯度洗脱,流速为1.0 mL/min,柱温30℃,检测波长为250 nm。结果:五味子醇甲保留时间约为13.8 min,五味子酯甲保留时间约为26.9 min,五味子醇甲、五味子酯甲与其他组分均能达到基线分离,分离度R>1.5,理论塔板数N>4 000。本试验方法简单、快速、准确,对南、北五味子通用,能满足一般实验室条件。结论:该试验方法可作为检测南、北五味子的含量是否符合《中国药典》要求的快速、实用测定方法。

Objective： To develop a simple,quick,accurate and general RP- HPLC method to simultaneously determine two major compounds in Schisandra chinensis（ Turcz.） Baill,namely Schizandrol A and Schisanther A. Methods： Chromatographic separations were carried out on a Agilent TC- C18（ 2） column（ 5 μm,4. 6 mm × 250 mm）,equipped with a UV detector at 250 nm,and the flow rate was 1. 0 mL / min. The mobile phase consisted of Methanol（ A） and water（ B）,with gradient elution,and the column temperature was maintained 30 ℃. Results： The retention time of two major compounds（ Schizandrol A and Schisanther A） was about 13. 8 min and 26. 9 min,respectively,with R 1. 5 and N 4 000. This method was simple, quick,accurate and could be generally applied to analyze the two major compounds in Schisandra sphenanthera Rehd. et Wils. and Schisandra chinensis Baill. Conclusion： The method established in this paper can be used as an effective and practical method to determine Schizandrol A and Schisantherain A quickly and simultaneously,and can be applied to evaluate the quality of Schisandrae and its compound preparation products.