稀土氟化钕微粒致大鼠肺损伤的实验研究

An Experimental Study of the Lung Injury in Rats Induced by Rare Earth Neodymium Fluoride Particles

目的:观察稀土氟化钕微粒对大鼠肺组织的氧化损伤及致纤维化作用。方法:采用非暴露式气管注入法对实验大鼠灌注不同剂量的氟化钕微粒悬浮液,用HE染色观察大鼠肺组织病理改变,分别采用黄嘌呤氧化酶法、硫代巴比妥酸法、羟脯氨酸碱性水解法测定肺组织匀浆中超氧化物歧化酶(SOD)活性、丙二醛(MDA)、羟脯氨酸(HYP)含量。结果:不同时间点各剂量组肺组织病理改变明显,且随染尘时间的延长肺组织呈纤维化趋势,不同剂量组肺组织病理改变未见明显差异。与对照组相比,染尘1、3个月时,各剂量组SOD活性明显降低,差异均有统计学意义;6个月时,只有高剂量组与对照组的差异有统计学意义。1个月时,各剂量组MDA含量高于对照组,差异均有统计学意义;3、6个月时,中、高剂量组与对照组的差异有统计学意义。1、3、6个月时,各剂量组HYP含量均高于对照组,除1个月低剂量组外,不同时间点各剂量组HYP含量与对照组比较差异均有统计学意义。结论:稀土氟化钕微粒可导致大鼠肺组织氧化损伤,随染尘时间的延长肺组织纤维化程度呈上升趋势。

Objective： To assess the oxidative- damage and fibrosis- causing effect of rare earth neodymium fluoride particles on the lung tissues in rats. Methods： Animal models were established by direct tracheal instillation of different doses of neodymium fluoride particle suspension into rat lungs non- surgically. Lung fibrosis in morphology was observed by HE staining. The superoxide dismutase（ SOD） activity,malondialdehyde（ MDA） content and Hydroxyproline（ HYP） content in the liver homogenate were assayed with xanthine oxidase method,thibabituric acid method and Hydroxyproline method,respectively. Results： The pulmonary damage was obvious at different time points in each dose group. There was an increase in the degree of lung fibrosis with the increase of dye dust time,but there was no remarkable difference in the degree of pulmonary damage among dose groups. Compared with that in the control group,the SOD activity in each dose group decreased significantly,one and three months after dye dust,with remarkable decrease only in the high dose group six months after dye dust; The MDA content in each dose group was higher than that in the control group,one month after dye dust,with MDA content in the medium and high dose groups significantly higher than that in the control group,three and six months after dye dust; The HYP content in each dose group was higher than that in the control group one,three and six months after dye dust; Except for low dose group one month after dye dust,the HYP content in each does group was significantly higher than that in the control group at different time points. Conclusion： Rare earth neodymium fluoride particles can cause some oxidative damage to the lung tissues in rats,and there is an increase in the degree of lung fibrosis in rats with the increase of dye dust time.