摘要
从我国使用的 19个主要香菇栽培菌种中制备了 2 5种原生质体单核体 ,以此为材料 ,以交配型基因为标记 ,在交配反应和RAPD两个水平上对这 19个菌株进行了较为详细的遗传相似性分析。 2 5种原生质体单核体两两配对共 30 0个组合 ,其中不亲和性反应为 110对 ,占配对总数的 36 .7%。根据交配反应结果 ,2 5个单核体可分为三大类群、7个交配型组。这些结果从交配型角度证明 ,这 19个菌株具有十分狭窄的以交配型基因为标记的遗传背景。RAPD反应和聚类分析中 ,这三大类群、7个交配型组基本上都能聚为一类 ,从而在分子水平上验证了交配型分析的结果 ,也为交配型基因在菌种鉴定中的作用提供了依据。
protoplasted monokaryons were prepared from 19 Lentinula edodes cultivating strains in China. Genetic similarity analysis of these 19 strains was carried out based on the mating reaction and RAPD analysis of 25 monokaryons with mating type genes as marker. It was found that the incompatibility reaction rate was 36.7% in 300 progenies from 25 protoplasted monokaryon pairings. Based on the mating reaction results, 25 protoplasted monokaryons could be divided into three classes with 7 mating type groups. And the results also indicated that these 19 strains had high genetic similarity. Similar results were obtained by RAPD analysis and cluster analysis. This implicated that mating type genes could be used for strain identification.
出处
《食用菌学报》
2000年第3期1-5,共5页
Acta Edulis Fungi
基金
国家自然科学基金资助项目 !编号 :39870 5 40
关键词
香菇
遗传相似性
交配型基因
RAPD
栽培菌种
Lentinula edodes
Genetic similarity
Protoplasted monokaryon
Mating type gene
RAPD
