姜黄素与HP-β-CD的包合作用机理及理化性质探讨

Inclusion Mechanism and Physicochemical Property of Curcumin and Hydroxypropyl-beta-cyclodextrin

采用光度法和Hildebrand-Benesi双倒数方程考察姜黄素与HP-β-CD的包合作用方式和作用机理。升高温度,姜黄素与HP-β-CD的包结常数增大,ΔrGm<0,ΔrHm>0,ΔrSm>0,表明其包结过程是一个自发、吸热的过程,其作用方式主要是一熵驱动下典型的疏水作用。Hildebrand-Benesi双倒数方程线性拟合较好,说明其包合比约为1∶1。据此在不同温度下制备姜黄素-HP-β-CD包合物,采用光谱法表征并验证,测得包合率均>98%,包合物的水溶性和稳定性较姜黄素均有改善。

The ultraviolet spectrophotometry and Hildebrand- Benesi double-reciprocal equation were used to investigate theinclusion mechanism and interaction fashion between curcu- rain with hydroxypropyl-beta-cyclodextrin （HP-β-CD）. As the temperature increased, the inclusion constants also increased. Furthermore,the inclusion process was spontaneous endother- mic one from the parameters such as △rGm 〈0,△rHm 〉0 and △rSm 〉 0,respectively. The inclusion interaction between cur- cumin and HP-β-CD was mainly a typical hydrophobic force driven by entropy. A good linearity from Hildebrand-Benesi double-reciprocal equation indicated the bonding ratio was 1：1 approximately. According to the above theory the inclu- sion complexes were prepared at different temperature and were eharaeterized by infrared spectroscopic analysis, respec- tively. In addition, the related physicochemieal properties of the inclusion complex were discussed preliminarily such as inclusion rate, water-solubility and stability, etc. The water- solubility and stability of the inclusion complex were all im- proved with comparison to eurcumin.