摘要
Pepper seedlings were hydroponically cultivated in 0-20 mg/L extraneous lanthanum chloride （La3＋） for different numbers of days to investigate its regulating effects under salt stress. The results showed that, depending on the salt stress of 0.75 g/L, a lower concentration of La3＋ （1.0 rag/L） might result in the lowest death rate, increase POD activity and change the band type of the stem and root on the 8th day. Furthermore, a methylation analysis by the methylation-sensitive amplified polymorphism （MSAP） technique indicated that a lower concentration of La3＋ （1.0 mg/L） triggered the lowest methylation level of 26.1%, of which the hypermethylation events were the primary type of methylation. In addition, the sequencing of 14 differ- entially expressed polymorphic fragments and the subsequent blast search revealed that La3＋ could induce methylation events in salt-tolerance sequences.
Pepper seedlings were hydroponically cultivated in 0-20 mg/L extraneous lanthanum chloride （La3＋） for different numbers of days to investigate its regulating effects under salt stress. The results showed that, depending on the salt stress of 0.75 g/L, a lower concentration of La3＋ （1.0 rag/L） might result in the lowest death rate, increase POD activity and change the band type of the stem and root on the 8th day. Furthermore, a methylation analysis by the methylation-sensitive amplified polymorphism （MSAP） technique indicated that a lower concentration of La3＋ （1.0 mg/L） triggered the lowest methylation level of 26.1%, of which the hypermethylation events were the primary type of methylation. In addition, the sequencing of 14 differ- entially expressed polymorphic fragments and the subsequent blast search revealed that La3＋ could induce methylation events in salt-tolerance sequences.
基金
Project supported by the Educational Commission Program of Anhui Province(KJ2013A074)
the National Xinghuo Plan(2012GA710028)
