期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

阿托伐他汀对ox-LDL诱导的人脐静脉内皮细胞中Wnt信号通路及相关因子表达的影响 被引量：1

Effects of Atorvastatin on Wnt Signaling Pathway and the Expression of Related Factors in ox-LDL Induced Human Umbilical Vein Endothelial Cells
原文传递
导出
摘要 目的:研究阿托伐他汀对氧化型低密度脂蛋白(ox-LDL)诱导的人脐静脉内皮细胞(HUVECs)中Wnt信号通路及相关因子Wnt1、β-连环蛋白(β-catenin)及蓬乱蛋白1(dvl-1)表达的影响。方法:将体外培养的HUVECs分为空白对照组、ox-LDL(50 mg/L)组和低、高剂量(ox-LDL 50 mg/L+阿托伐他汀0.5、10μmol/L)组,分别加入相应药物培养24 h后,用硝酸还原酶法测定各组细胞中一氧化氮(NO)含量,用免疫组化法和蛋白质印迹法检测各组细胞中Wnt1、β-catenin及dvl-1蛋白的表达。结果:与空白对照组比较,其他3组细胞中NO含量明显减少(P<0.01),Wnt1、β-catenin、dvl-1蛋白表达均明显增强(P<0.01);与ox-LDL组比较,低、高剂量组细胞中NO含量均明显增加(P<0.01),Wnt1、β-catenin、dvl-1蛋白表达均明显减弱(P<0.01)。结论:阿托伐他汀能抑制ox-LDL诱导的HUVECs中Wnt信号通路及相关因子表达,其可能是阿托伐他汀抗动脉粥样硬化的机制之一。 OBJECTIVE： To study the effects of atorvastatin on Wnt signaling pathway and the expression of related factors Wntl, β-catenin and dvl-1 in oxidized low density lipoprotein （ox-LDL） induced human umbilical vein endothelial cells （HU- VECs）. METHODS： HUVECs cultred in vitro were divided into blank control group, ox-LDL group （50 mg/L）, low-dose and high-dose groups （ox-LDL 50 mg/L＋atrovastatin 0.5, 10 μmol/L）. They were cultured with relevant drugs for 24 h; nitrate reduc- tase method was adopted to determine the content of NO; and Wntl, β-catenin and dvl-1 protein expressions were determined by immunohistochemical method and Western blotting assay. RESULTS： Compared with blank control group, the content of NO de- creased significantly in other 3 groups （P〈0.01）, while Wntl, β-catenin and dvl-1 protein expressions increased significantly （P〈 0.01） ; compared with ox-LDL group, the content of NO in ox-LDL low-dose and high-dose groups increased significantly （P〈 0.02）, while Wntl, β-catenin and dvl-1 protein expressions decreased significantly （P〈0.01）. CONCLUSIONS： Atorvastatin can inhibit Wnt signaling pathway and the expression of related factors in ox-LDL induced HUVECs, which may be one of anti-athero- sclerosis mechanisms of atorvastatin.
作者 李艳伟 于晓玲 申玉超
机构地区 辽宁医学院附属第一医院老年病科高级医疗服务中心 沧州市中心医院重症医学科
出处 《中国药房》 CAS CSCD 2014年第17期1574-1577,共4页 China Pharmacy
关键词 阿托伐他汀 人脐静脉内皮细胞 WNT信号通路 氧化型低密度脂蛋白 Atorvastatin Human umbilical vein endothelial cells Wnt signaling pathways Oxidized low density lipoprotein
分类号 R965 [医药卫生—药理学]
  • 相关文献

参考文献14

  • 1Schwartz BG,Economides C,Mayeda GS,et al.The en-dothelial cell in health and disease:its function,dysfunc-tion,measurement and therapy[J].Int J Impot Res,2010,22(2):77.
  • 2王育林,江淼,赵欣,程绪杰,闫继峰,杨向军,宋建平,蒋延波,惠杰,李勋,刘志华,蒋文平.β-钙联蛋白在兔腹主动脉粥样硬化斑块中的表达[J].中华实验外科杂志,2006,23(9):1100-1100. 被引量：2
  • 3张秀梅,于晓玲,申玉超,骆振华.阿托伐他汀对ox-LDL诱导的人脐静脉内皮细胞缝隙连接的影响[J].中国药房,2013,24(5):412-415. 被引量：3
  • 4Ross R.Atherosclerosis:an inflammatory disease[J].N En-gl J Med,1999,340(2):115.
  • 5Murphy JE,Vohra RS,Dunn S,et al.Oxidised LDL in-temalisation by the LOX-1 scavenger receptor is depen-dent on a novel cytoplasmic motif and is regulated by dy-namin-2[J].J Cell Sci,2008,121(Pt 13):2 136.
  • 6Frangogiannis NG.Chemokines in ischemia and reperfus-ion[J].Thromb Haemost,2007,97(5):738.
  • 7Sawamura T,Kume N,Aoyama T,et al.An endothelial receptor for oxidized low-density lipoprotein[J].Nature,1997,386(6 620):73.
  • 8Xing Y,Clements WK,Kimelman D,et al.Crystal struc-ture of a beta-catenin/axin complex suggests a mechanism for the beta-catenin destruction complex[J].Genes Dev,2003,17(22):2 753.
  • 9Giles RH,van Es JH,Clevers H.Caught up in a Wnt storm:Wnt signaling in caner[J].Biochim Biophys Acta,2003,1 653(1):1.
  • 10Birchmeier W.Cell adhesion and signal transduction in cancer:conference on cadherins,catenins and cancer[C].EMBO Rep,2005,6(5):413.

二级参考文献9

  • 1戴萍,金蜀蓉.他汀类药物的非调脂作用研究进展[J].中国药房,2005,16(12):949-952. 被引量：15
  • 2石翠格,胡刚,汪海.天然药物槟榔碱对氧化低密度脂蛋白致血管内皮细胞损伤的保护作用研究[J].科学技术与工程,2007,7(12):2780-2783. 被引量：15
  • 3Morel S, Burnier L, Kwak BR.Connexins participate in the initiation and progression of atherosclerosis[J]. Semin Im- munopathol, 2009,31 ( 1 ) : 49.
  • 4Itabe H. Oxidative modification of LDL: its pathological role in atherosclerosis[J]. Clin Rev Allergy Immunol, 2009,37(1) :4.
  • 5Tabas I, Williams K J, Boren J. Subendothelial lipoprotein retention as the initiating process in atherosclerosis: up- date and therapeutic implications[J]. Circulation, 2007,116(16):1 832.
  • 6Kwak BR, Veillard N, Pelli G, et al. Reduced Connexin 43 expression inhibits atherosclerotic lesion formation in- low-density lipoprotein receptor-deficient mice[J]. Circu- lation,2003,107(7) : 1 033.
  • 7Chadjichristos CE, Scheckenbach KE, Van veen TA, et al. Endothelial-specific deletion of Cormexin 40 promotes atherosclerosis by increasing CD73-dependent leukocyte adhesion[J].Circulation, 2010,121 ( 1 ) : 123.
  • 8Wang LH, Chen JZ, Sun YL, et al. Statins reduce Connex- in 40 and Connexin 43 expression in atherosclerotic aorta of rabbits [J]. lnt J Cardiol, 2005,100 (3) : 467.
  • 9Linhares VL, Almeida NA, Menezes DC, et al. Transcrip- tional regulation of the murine Connexin40 promoter by cardiac factors Nkx2-5, GATA4 and TbxS[J], Cardiovasc Res, 2004,64 (3) :402.

共引文献3

同被引文献28

  • 1Sardar P, Udell JA, Chatterjee S, et al. Effect of intensive versus standard blood glucose control in patients with type 2 diabetes mellitus in different regions of the world: systematic review and meta-analysis of randomized controlled trials [ J ]. J Am Heart Assoc, 2015, 4(5).
  • 2Stubhs B, Vancampfort D, De Herr M, et al. The prevalence and predictors of type two diabetes mellitas in people with schizophrenia: a systematic review and comparative meta-analysis [J]. Acta Psychiatr Scand, 2015.
  • 3Song H, Wu F, Zhang Y, et al. Irisiu promotes human umbilical vein endothelial cell proliferation through the ERK signaling pathway and partly suppresses high glucose-induced apoptosis [J]. PLoS One, 2014, 9(10): e110273.
  • 4Hou Q, Lei M, Hu K, et al. The effects of high glucose levels on reactive oxygen species-induced apoptosis and involved signaling in human vascular endothelial cells [ J]. Cardiovasc Toxicol, 2015, 15(2) : 140 -146.
  • 5Wassmann S, Laufs U, Baumer AT, et al. HMG-CoA reductase inhibitors improve endothelial dysfunction in normocholesterolemic hypertension via reduced production of reactive oxygen species [ J]. Hypertension, 2001, 37 (6) : 1450 - 1457.
  • 6Li P, Guo X, Lei P, et al. PI3K/Akt/uncoupling protein 2 signaling pathway may be involved in cell senescence and apoptosis induced by angiotensin II in human vascular endothelial cells [J]. MolBio!Rep, 2014, 41(10): 6931 -6937.
  • 7Chen HF, Liu SJ, Chen G. Heat shock protein 27 phosphorylation in the proliferation and apoptosis of human umbilical vein endothelial cells induced by high the phosphoinositide 3kinase/Akt and glucose through signalregulated kinase 1/2 pathways [J]. Mol Med extracellular Rep, 2015,11(2): 1504 -1508.
  • 8Sheu ML, Ho FM, Yang RS, et al. High glucose induces human endothelial cell apoptosis through a phosphoinositide 3-kinase- regulated eye|ooxygenase-2 pathway [ J ]. Arterioseler Thromb Vase Biol, 2005, 25 (3) : 539 - 545.
  • 9Madonna R, De Caterina R. Prolonged exposure to high insulin impairs the endothelial PI3-kinase/Akt/nitric oxide signalling [ J]. Thromb Haemost, 2009, 101 (2) : 345 - 350.
  • 10Yuan X, Chen K, He H, et al. [ Effect of GLP-1 on high glucose-induced human umbilical vein endothelial cell apoptosis and mechanism ] [ J ]. Zhongnan Daxue Xuebao Yixue Ban, 2013, 38(10) : 1029 - 1034.

引证文献1

二级引证文献6

中国药房
2014年 第17期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部