摘要
目的:建立测定生脉注射液中重金属元素铜、砷、镉、汞、铅含量的方法。方法:采用电感耦合等离子体质谱法。射频入射功率为1 550 W,萃取电压为-143 V,采样深度为62 mm,载气为高纯氩气,流速为1.1 L/min。经微波消解后的生脉注射液,以钪、锗、铟、铋为内标,以茶叶作为质控标准物质,测定样品中铜、砷、镉、汞、铅的含量。结果:各元素在各自的检测质量浓度范围内线性关系良好(r均不低于0.999 8),回收率均大于90%,RSD均不大于2.8%(n=3),铜、砷、镉、汞、铅检测限分别为0.10、0.06、0.03、0.008、0.02 ng/ml。各批样品中重金属含量均在合格范围内。结论:本方法操作简便、准确可靠、专属性强,可用于生脉注射液中重金属元素的含量测定。
OBJECTIVE: To establish the method for the content determination of heavy metals in Shengmai injection as Cu, As, Cd, Hg and Pb. METHODS: ICP-MS was adopted with incident power of 1 550 W, extraction voltage of --143 V, sampling depth of 62 nm; high purity argon was used as carrier at the flow rate of 1.1 L/min. After Shengmai injection digested by micro- wave, the contents of Cu, As, Cd, Hg and Pb were determined by using scandium, germanium, indium and bismuth as internal standard, tea leaf as standard substance of quality control. RESULTS: The calibration curve of heavy metals was in linear relationship (r≥0.999 8) with recovery〉90% (RSD≤2.8%, n=3). The detection limits of Cu, As, Cd, Hg and Pb were 0.10, 0.06, 0.03, 0.008 and 0.02 ng/ml, respectively. The contents of heavy metals were all in an acceptable range. CONCLUSIONS: The method is simple, accurate, reliable and specific, and it can be used for the content determination of heavy metal in Shengmai injection.
出处
《中国药房》
CAS
CSCD
2014年第17期1619-1621,共3页
China Pharmacy