ICP-MS法测定生脉注射液中5种重金属元素的含量

Content Determination of 5 Heavy Metals in Shengmai Injections by ICP-MS

目的:建立测定生脉注射液中重金属元素铜、砷、镉、汞、铅含量的方法。方法:采用电感耦合等离子体质谱法。射频入射功率为1 550 W,萃取电压为-143 V,采样深度为62 mm,载气为高纯氩气,流速为1.1 L/min。经微波消解后的生脉注射液,以钪、锗、铟、铋为内标,以茶叶作为质控标准物质,测定样品中铜、砷、镉、汞、铅的含量。结果:各元素在各自的检测质量浓度范围内线性关系良好(r均不低于0.999 8),回收率均大于90%,RSD均不大于2.8%(n=3),铜、砷、镉、汞、铅检测限分别为0.10、0.06、0.03、0.008、0.02 ng/ml。各批样品中重金属含量均在合格范围内。结论:本方法操作简便、准确可靠、专属性强,可用于生脉注射液中重金属元素的含量测定。

OBJECTIVE： To establish the method for the content determination of heavy metals in Shengmai injection as Cu, As, Cd, Hg and Pb. METHODS： ICP-MS was adopted with incident power of 1 550 W, extraction voltage of --143 V, sampling depth of 62 nm; high purity argon was used as carrier at the flow rate of 1.1 L/min. After Shengmai injection digested by micro- wave, the contents of Cu, As, Cd, Hg and Pb were determined by using scandium, germanium, indium and bismuth as internal standard, tea leaf as standard substance of quality control. RESULTS： The calibration curve of heavy metals was in linear relationship （r≥0.999 8） with recovery〉90% （RSD≤2.8%, n=3）. The detection limits of Cu, As, Cd, Hg and Pb were 0.10, 0.06, 0.03, 0.008 and 0.02 ng/ml, respectively. The contents of heavy metals were all in an acceptable range. CONCLUSIONS： The method is simple, accurate, reliable and specific, and it can be used for the content determination of heavy metal in Shengmai injection.