子宫颈石蜡包埋样本检测HPV E6/E7mRNA用于子宫颈癌筛查的价值

The application value of HPV E6/E7 mRNA detection in paraffin-embedded samples for cervical cancer screening

目的 探讨检测石蜡包埋样本HPV E6/E7 mRNA在子宫颈癌筛查及预后判断中的应用价值.方法 应用支链DNA技术对石蜡包埋样本行HPV E6/E7 mRNA定量检测,以病理学诊断结果为标准分组并对拷贝值进行分析.比较各组间HPV E6/E7 mRNA阳性率及拷贝均数.结果 子宫颈癌组的HPVE 6/E7 mRNA阳性率高于子宫颈上皮内瘤变（CIN）1、CIN2、CIN3组、慢性子宫颈炎组[分别为94.29％（66/70）、30.30％（20/66）、50.00％（19/38）、59.46％（44/74）、5.66 ％（3/50）,均P＜ 0.004 5],CIN组的阳性率均高于慢性子宫颈炎组（P＜0.004 5）.CIN2组和CIN3组的阳性率均高于CIN1组（P＜0.0045）,CIN2组和CIN3组间的阳性率差异无统计学意义（P＞0.004 5）.子宫颈癌组的拷贝值高于慢性子宫颈炎组、CIN1、CIN2、CIN3组（分别为16 508.730±8 741.402、929.337±460.880、3 239.681±1 447.399、5 286.224±2 933.445、4.941±2.263,均P＜0.05）,CIN组的拷贝值均高于慢性子宫颈炎组（P＜0.05）,CIN3组的拷贝数值高于CIN1组（P＜0.05）,CIN1组和CIN2组、CIN2组和CIN3组间的拷贝值差异均无统计学意义（P＞0.05）.结论 HPV E6/E7 mRNA阳性率和拷贝值的变化趋势与子宫颈病变的程度有关.子宫颈石蜡包埋组织检测HPV E6/E7 mRNA在预见疾病风险上有较高的临床应用价值,可作为子宫颈癌筛查和指导随访的有效措施.

Objective To explore the application value of detecting HPV E6/E7 mRNA in cervical cancer screening and prognosis.Methods bDNA technology was used to detect the HPV E6/E7 mRNA levels in 301 cases of paraffin-embedded cervical samples that were grouped based on pathological diagnosis.Statistic differece between multiple groups were assessed by chi-square segmentation method.Results The positive detection rate of HPV E6/E7 mRNA is higher in cervical cancer group than that in CIN1-3 groups and cervicitis group,which were 94.29 % （66/70）,30.30 % （20/66）,50.00 % （19/38）,59.46 % （44/74）,5.66 % （3/50）,respectively （all P 〈 0.004 5）.The HPV E6/E7-positive rates in all three CIN groups were higher than that in cervicitis group （P 〈 0.004 5）.The positive rates in CIN2 and CIN3 group were higher than that in CIN1 group （P 〈 0.004 5）.HPV E6/E7 mRNA levels in cervical cancer group were higher than those in CIN groups and cervicitis group （16 508.730±8 741.402,929：337±460.880,3 239.681±1 447.399,5 286.224±2 933.445,4.941±2.263,respectively,all P 〈 0.05）.CIN groups had higher copy numbers of HPV E6/E7 mRNA than cervicitis group （P 〈 0.05）.More copies of HPV E6/E7 mRNA were detected in CIN3 group than those in CIN1 group （P 〈 0.05）.Conclusions The positive rate and expression levels of HPV E6/ E7 mRNA was related to the severity of cervical diseases.HPV E6/E7 mRNA detection in paraffin-embedded samples have a high clinical application value in predicting the risk of cervical cancer.It＆#39; s an effective measure for cervical cancer screening and follow-up.