IL-10基因启动子多态性与强迫症关系

Association of interleukin 10 gene promoter polymorphisms and obsessivecompulsive disorder

目的探讨白细胞介素-10（IL-10）基因启动子多态性与强迫症（OCD）的关系。方法381例OCD患者和485名健康对照为研究对象，通过聚合酶链式反应与限制性片段长度多态性（PCR—RFLP）基因分型技术对IL-10基因标签单核酸多态性（SYP）位点592A／C（rs1800872）、1082A／G（rs1800896）、819T／C（rs1800871）进行基因分型；以耶鲁一布朗强迫量表（Y-BOCS）评定OCD患者的病情。结果OCD组和对照组IL-10基因rs1800872、rs1800896、rs1800871位点在基因型频率分布与等位基因频率分布差异均无统计学意义（P〉0．05）；不同发病年龄女性3个SNPsOCD组与对照组人群基因型与等位基因频率分布无明显差异（P〉0．05）；男性rs1800896基因型（X2=6．51，P=0．039）和等位基因（X2=6．59，P=0．01，OR=1．84，95％CI=1．15—2．95）频率分布在OCD组与对照组之间存在差异；OCD组男女性别之间rs1800896基因型（矿=8．08，P=0．02）和等位基因（X2==6．97，P=0．008，OR：1．95，95％CI：1．18—3．23）频率分布也存在差异。结论IL-10基因（rs1800896）可能与男性OCD易感性有关。

Objective To investigate the association between interleukin 10 （IL-10）gene promoter polymorphism and obessive-compulsive disorder（OCD）. Methods Genotyping for IL-10 gene SNP592A/C （ rs1800872）, 1082 A/G （rs1800896） ,and 819T/C（ rs1800871 ）were performed for 381 OCD patients and 485 health controls with polymerase chain reaction and restriction fragment length polymorphism techniques. The OCD patients were assessed with Yale- Brown Obsessive-Compulsive Scale （Y-BOCS）. Results There were no significant differences in the frequency distribu- tion of genotype and allele of IL-10 gene（ rs1800872 ,rs1800896 ,and rs1800871 ）between OCD patients and health con- trols（ P 〉 0. 05 ）. There were no significant differences in the freqency distribution of genotype and allele of 3 single nu- cleotide polymorphisms （SNPs） for the females of different onset age between OCD group and control group（ P 〉 0. 05 ）. There were significant differences in the freqency distribution of genotype （X2 = 6. 51, P = 0. 039 ） and allele （X2 = 6. 59, P = 0.01 ; odds ratio [ OR ] = 1.84,95 % confidence interval [ 95 % CI ] = 1.15 - 2.95 ） of IL- 10 gene （ rs 1800896 ） for the males between OCD patients and health controls ,and there were significant differences in the freqency distribution of geno type（x2 =8.08,P =0.02）and allele（x2 =6.97,P = 0.008;OR = 1.95,95% CI= 1.18 -3.23）of IL-10 gene （rs1800896）between female OCD group and male OCD group. Conclusion The results support a role for IL-10 gene （rs1800896） in conferring susceptibility to OCD in male.