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Allele-specific DNA methylation analyses associated with siRNAs in Arabidopsis hybrids 被引量：1

Allele-specific DNA methylation analyses associated with siRNAs in Arabidopsis hybrids
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摘要 Accumulating evidence has suggested that epigenetic marks including DNA methylation,small RNA and histone modification may involve hybrid vigor in plants.However,knowledge about how epigenetic marks in hybrids regulate gene expression is still limited.Based on genome-wide DNA methylation landscapes of Arabidopsis thaliana Ler and C24 ecotypes and their reciprocal F1 hybrids which were obtained in our previous work,we analyzed allele-specific DNA methylation and distinguished cis-and trans-regulated DNA methylation in hybrids.Our study indicated that both cis-and trans-regulated DNA methylation played roles in hybrids,when cis-regulation played a major role in CG methylation and trans-regulation played major roles in CHG and CHH methylation.In addition,we observed correlations between trans-regulated DNA methylation and siRNA densities.Enriched siRNA regions were significantly concurrent with highly trans-regulated DNA methylation regions.Our results illustrated DNA methylation regulation patterns integrated with siRNAs in Arabidopsis hybrids,and shed light on understanding the mechanism of epigenetic reprogramming for hybrid vigor. Accumulating evidence has suggested that epigenetic marks including DNA methylation,small RNA and histone modification may involve hybrid vigor in plants.However,knowledge about how epigenetic marks in hybrids regulate gene expression is still limited.Based on genome-wide DNA methylation landscapes of Arabidopsis thaliana Ler and C24 ecotypes and their reciprocal F1 hybrids which were obtained in our previous work,we analyzed allele-specific DNA methylation and distinguished cis-and trans-regulated DNA methylation in hybrids.Our study indicated that both cis-and trans-regulated DNA methylation played roles in hybrids,when cis-regulation played a major role in CG methylation and trans-regulation played major roles in CHG and CHH methylation.In addition,we observed correlations between trans-regulated DNA methylation and siRNA densities.Enriched siRNA regions were significantly concurrent with highly trans-regulated DNA methylation regions.Our results illustrated DNA methylation regulation patterns integrated with siRNAs in Arabidopsis hybrids,and shed light on understanding the mechanism of epigenetic reprogramming for hybrid vigor.
作者 CHEN ShaoXia HE Hang DENG XingWang
机构地区 State Key Laboratory of Protein and Plant Gene Research Department of Molecular
出处 《Science China(Life Sciences)》 SCIE CAS 2014年第5期519-525,共7页 中国科学（生命科学英文版）
基金 supported by Ministry of Science and Technology of China(2013CBA01402) Ministry of Science and Technology of China(2012CB910900) Ministry of Agriculture of China(2010ZX08010-003) Peking University 985 Program
关键词 DNA甲基化 siRNA 混合动力车 甲基化分析 等位基因 拟南芥 调控基因表达 表观遗传 SNP, DNA methylation, siRNA, cis-, trans-, allele
分类号 Q943.2 [生物学—植物学]
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Science China(Life Sciences)
2014年 第5期

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