摘要
本文以富含酚类物质的牡丹叶片为材料,就 CTAB 法提取植物组织中的 DNA 的方法做了进一步的改进,主要涉及二巯基乙醇的用量、氯仿/异戊醇的用量、以及 DNA 晾干方法的改进等,为后续的分子生物学试验提供了纯净的基因组 DNA。
Taking peony leaves full of phenol as experimental materials,the improvement of extracting DNA from plant tissues by CTAB,which involves doses of 2-thiclethylal-cohol chloroform/isoamy- lalcohol and improvement of drying DNA in the air,supplies DNA of purified genes for the folliwing molecular biological exeriements.
