酯化法提取生物基丁二酸

Extraction of Bio-Based Succinic Acid by Esterification Method

生物法获得的丁二酸发酵液中,含有部分残留底物、副产物以及无机离子等杂质,不利于其分离纯化。探讨了丁二酸发酵液经酸化与酯化分离纯化生物基丁二酸的过程。正交实验优化的酯化工艺为:丁二酸盐为丁二酸二钠、氢离子与丁二酸二钠的物质的量比为2.2∶1、反应时间12h,在此条件下,生物基丁二酸收率为91.7%。Box-Behnken中心组合设计实验优化的纯化工艺为:活性炭加量3.3%、脱色温度72℃、脱色时间37min,在此条件下,生物基丁二酸纯度达98.44%。通过红外光谱、X-射线衍射分析确证提取物质为生物基丁二酸,利用扫描电子显微镜观察酯化法提取的生物基丁二酸的微观形貌,为生物发酵法产丁二酸的产业化奠定了基础。

Fermentation mixture of succinic acid production contains residual substrate,by-products and inorganic ions that hinder downstream separation and purification. The separation and purification of bio-based succinic acid from fermentation mixture by acidification and esterificafion were investigated. The esterification conditions were determined using orthogonal experiment as follows.the molar ratio of hydrogen cation to diso- dium succinate of 2.2 ＂ 1,the reaction time of 12 h. The yield of bio-based succinic acid was 91.7~. The purifi- cation conditions were determined using Box-Behnken central composite design experiment as follows.activated carbon addition amount of 3.3%, decoloring temperature of 72℃,decoloring time of 37 rain. The purity of bio- based succinic acid was 98.44%. The structure of bio-based succinic acid was characterized by IR, XRD, SEM. This study lays the foundation for industrialization of production of bio-based succinic acid by fermentation.