中晚期鼻咽癌患者CD4^+CD25^+T调节细胞和Th17细胞的免疫病理意义

Immunopathological Significance of CD4^+CD25^+Regulatory T Cells and Th17 Cells in Patients with Intermiddle and Advanced Stage Nasopharyngeal Carcinoma

目的探讨中晚期鼻咽癌患者外周血CD4+CD25+T调节细胞和Th17细胞的免疫病理意义。方法采用流式细胞术、RT-PCR方法检测15例中晚期鼻咽癌患者外周血CD4+CD25+T调节细胞、Th17细胞比例和Foxp3、ROR-γt转录活性,并与10例健康体检者相应指标进行比较,分析其免疫病理意义。结果中晚期鼻咽癌患者外周血CD4+CD25+T调节细胞比例及Foxp3转录活性明显高于健康体检者(P<0.05);而Th17细胞比例及ROR-γt转录活性低于健康体检者(P<0.05)。结论 CD4+CD25+T调节细胞、转录因子Foxp3和Th17细胞比例、转录因子ROR-γt共同构成一个平衡反馈体系,参与中晚期鼻咽癌患者肿瘤微环境中免疫耐受现象的发展及免疫逃逸的发生过程。

Objective To investigate the immunopathological significance of CD4 ＋ CD25 ＋ regulatory T cells and Th17 cells in peripheral blood of the patients with intermiddle and advanced stage nasopharyngeal carcinoma. Meth-ods In this study,15 cases of intermiddle and advanced stage nasopharyngeal carcinoma of the observation group and 10 healthy persons of the control group were included to detect their ratio of CD4 ＋ CD25 ＋ T cells and Th17 cells in the peripheral blood by flow cytometry technique,transcriptional activity of Foxp3 and ROR-γt by RT-PCR procedure. Then,a comparative analysis was made to explore their implications in the immunopathological correlation of nasopharyngeal carcinoma. Results The ratio of CD4 ＋ CD25 ＋ T cells to total CD4 ＋ T cells and transcriptional activity of Foxp3 in the peripheral blood among the patients with intermiddle and advanced stage nasopharyngeal car-cinoma were significantly higher than those in the healthy persons（P ＆lt; 0. 05）. Meanwhile,the ratio of Th17 cells to total CD4 ＋ T cells and transcriptional activity of ROR-γt in the peripheral blood among the patients with intermiddle and advanced stage nasopharyngeal carcinoma was significantly lower than those in the healthy persons（P ＆lt; 0. 05）. Conclusion It may be true that CD4 ＋ CD25 ＋ T cells,transcriptiona regulatory factor Foxp3,and Th17 cells as well as ROR-γt altogether compose of a regulating system in a balance feedback way to evolve the developing process of＆amp;nbsp;immune tolerance phenomena in the microtumor enviroment and the implication of immune escape among patients with intermiddle and advanced stage nasopharyngeal carcinoma.