Axin蛋白表达与肝细胞癌发生及预后关系的研究

Study on the expression of Axin correlates with genesis and prognosis of hepatocellular carcinoma

目的观察Axin蛋白在正常肝组织、肝细胞癌组织、癌旁组织中的表达情况,以及其表达变化与肝细胞癌的发生及与预后影响因素间的关系。方法选取术前未行放疗、化疗,经手术切除的患者标本40例,结合相应的临床及病理学资料,采用免疫组化法(SABC法)检测正常肝组织、肝细胞癌及其癌旁组织中Axin蛋白表达情况及定位。结果 Axin蛋白在正常肝组织、肝细胞癌组织及癌旁组织中均有表达,阳性表达率分别是85.7%、52.5%和77.5%。Axin蛋白的阳性表达定位于细胞胞质中;正常肝组织与癌旁组织中的阳性表达差异无显著性(P>0.05);正常肝组织与癌组织、癌旁组织与癌组织中Axin蛋白表达比较差异有显著性(P<0.05);Axin在肝细胞癌组织的表达与患者的性别、年龄、肿瘤、大小、包膜形成、AFP表达水平等无关(P>0.05),而与肝细胞癌(HCC)的门静脉癌栓形成、临床TNM分期相关(P<0.05)。结论 (1)Axin蛋白在HCC组织、癌旁组织、正常肝组织中均有表达,但三者间差异有显著性,HCC组织中阳性表达率最低,癌旁组织次之,正常肝组织中最高。(2)Axin蛋白表达减弱可能参与肝细胞癌的发生;Axin蛋白表达水平可作为肝细胞癌患者诊断和预后判断的一项指标。

Objective To detect the expression and distribution of Axin in the normal liver tissue , paracarcinomatous tissue and primary hepatocellular carcinoma （HCC） tissue and investigate the rela-tionship between Axin protein expression and the genesis ,and prognosis of HCC .Methods The ex-pression of Axin protein was evaluated in paraffin embedded tissues by immunohistochemical tech-niques （SABC） in 40 cases of HCC ,40 cases of paracarcinomatous tissue and 14 cases of normal liver tissue .And 14 cases of normal stomach tissue were evaluated as a positive control .Results The ex-pression of Axin protein was observed in normal liver tissues ,paracarcinomatous tissues and HCC tis-sues ,and the positive expressions of Axin were 85 .7% ,77 .5% ,52 .5% respectively .The brown granules of positive cell were mainly distributed in the cytoplasm .There were no statistical significant-ly differences in the expression of Axin was found between normal liver tissue and paracarcinomatous tissue （P＆gt;0 .05） ,but there were statistical significantly differences between the paracarcinomatous tissue and HCC （P＆lt;0 .05） .Axin protein expression were not related to sex ,age ,tumor size and the expression of AFP （P＆gt;0 .05） ,but were significantly correlated with portal vein invasion and clinical pathological stage （P＆lt;0 .05） .In normal stomach tissue ,the expression of Axin was strong in the stem-cells and weak in the mature cells .But its expression in normal liver tissue was well-propori-oned .Conclusion Axin expresses mainly in the stem-cells ,and its expression is important to sustain the function of the stem-cell：Hepatitis B and liver cirrhosis have no influence to the expression of Ax-in .The reduced expression of Axin correlates with the genesis of HCC .The check of its expression＆amp;nbsp;can be used as an independently index to evaluate the biological behavior and prognosis of HCC .