淫羊藿黄酮类组分表观溶解度和油水分配系数的测定

Determination of Apparent Solubility and Oil/water Partition Coefficient of Flavonoid Components from Epimedii Folium

目的：测定淫羊藿黄酮类组分的表观溶解度和油水分配系数，为该组分整体水溶性和脂溶性的表征提供参考。方法：以淫羊藿黄酮类组分为模型药物，采用HPLC测定朝藿定A，B，C及淫羊藿苷在不同缓冲液中的平衡溶解度和表观油水分配系数（P_app），流动相乙腈-水（25∶75），检测波长270 nm。结果：朝藿定A，B，C和淫羊藿苷在不同pH缓冲液中的整体平衡溶解度顺序为朝藿定B〉朝藿定A〉朝藿定C〉淫羊藿苷，P_app总体变化趋势均为先变大后变小再变大，油水分配系数-1.437-3.147。结论：淫羊藿黄酮类组分的水溶性及脂溶性较好，在不同pH缓冲液中变化趋势相近，朝藿定B属于微解，朝藿定A，C和淫羊藿苷属于极微溶解。

Objective： To determine apparent solubility and apparent oil/water partition coefficient of flavonoid components from Epimedii Folium,so as to lay foundation for overall solubility characterization of these components. Method： Taking flavonoid components from Epimedii Folium as model drug,HPLC was adopted to determine equilibrium solubilities and apparent oil/water partition coefficients（P_app） of epimedin A,B,C and icariin in different pH buffers by taking mobile phase of acetonitrile-water（25：75） and detection wavelength at 270 nm. Result： Overall equilibrium solubilities of flavonoid components from Epimedii Folium in different pH buffers were in order of epimedin B〉epimedin A〉epimedin C〉icariin,oil/water partition coefficients were -1.437-3.147,overall trends of P_app were changed from small to large and then to small,to large at last. Conclusion： Water-soluble and fat-soluble of flavonoid components from Epimedii Folium were well with a similar overall trend,epimedin B belonged to slightly soluble,epimedin A,C and icariin belonged to very slightly soluble.