UPLC测定香鳞毛蕨中绵马素BB的含量

Determination of Aspidin BB in Dryopteris fragrans by UPLC

目的：建立超高效液相色谱法测定香鳞毛蕨药材中绵马素 BB的含量。方法：采用XBridge^TM BEH C_18（4.6 mm100 mm，2.5 μm）色谱柱，以乙腈-0.1%三氟乙酸水溶液（90∶10）为流动相，流速0.80 mL·min^-1，检测波长290 nm，柱温40 ℃，进行测定。结果：绵马素BB在0.050 28-0.301 68 μg线性关系良好（r=0.999 8），平均回收率为98.87%（RSD 0.44%）。结论：该方法快速、准确、重复性好，可用于香鳞毛蕨的含量测定和质量控制。

Objective： To establish an UPLC method for the content determination of aspidin BB in Dryopteris fragrans. Method： Determination was performed on XBridge^TM BEH C_18 column （4.6 mm100 mm,2.5 μm） with mobile phase consisted of acetonitrile-0.1% trifluoroacetic acid with detection wavelength at 290 nm. The flow rate was set to 0.8 mL·min^-1,while the column temperature was set at 40 ℃. Result： The linear range of aspidin BB was 0.050 28-0.301 68 μg（r=0.999 8）with an average recovery of 98.87%（RSD 0.44%,n=6）. Conclusion： The method is fast,accurate and reproducible, which can be used to control the quality of D. fragrans.