摘要
目的:建立同时测定向日葵列当中crenatoside和类叶升麻苷含量的HPLC法。方法:采用反相高效液相色谱法,用Agilent ZORBAX SB-C18 (4.6 mm250 mm,5 μm)柱,以乙腈-0.01%冰醋酸溶液(18∶82) 为流动相,流速1.0 mL·min^-1,检测波长330 nm,柱温30 ℃,在30 min内分离检测了这两种化合物。结果:RP-HPLC测定的crenatoside和类叶升麻苷线性范围分别在在1.08-16.2 μg(r=0.999 9)和1.00-15.0 μg(r=0.999 8),平均加样回收率分别为97.23%,96.27%,RSD分别为1.21%,1.66%。结论:该方法简便,精密度、重复性良好,结果准确可靠,可以作为向日葵列当及其相关制剂的质量控制。
Objective: To establish a RP-HPLC method for simultaneous determination of phenylethanoid glycosides crenatoside and acteoside in Orobanche cumana. Method: The analysis was performed on a Agilent ZORBAX SB-C18 column (4.6 mm250 mm,5 μm) with CH3CN-0.01%HOAc(18:82) as mobile phase at a flow rate of 1.0 mL·min^-1and at a column temperature of 30 ℃,330 nm as the detection wavelength,the well separation was achieved in 30 min. Result: Crenatoside showed good relationship at the range of 1.08-16.2 μg(r=0.999 9),and acteoside at the range of 1.00-15.0 μg (r=0.999 8). The average recovery were 97.23% and 96.27%,relative standard derivation (RSD) of 1.21% and 1.66% for crenatoside and acteoside. Conclusion: The proposed method is simple,rapid,accurate and reliable.It can be used for the quality evaluation of O. cumana and its preparation.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第10期87-89,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
吉林市科技计划项目(201162512)