RP-HPLC同时测定向日葵列当中苯乙醇苷类化合物crenatoside和类叶升麻苷

Simultaneous Determination of Crenatoside and Acteoside in Orobanche cumana by RP-HPLC

目的：建立同时测定向日葵列当中crenatoside和类叶升麻苷含量的HPLC法。方法：采用反相高效液相色谱法，用Agilent ZORBAX SB-C18 （4.6 mm250 mm，5 μm）柱，以乙腈-0.01%冰醋酸溶液（18∶82） 为流动相，流速1.0 mL·min^-1，检测波长330 nm，柱温30 ℃，在30 min内分离检测了这两种化合物。结果：RP-HPLC测定的crenatoside和类叶升麻苷线性范围分别在在1.08-16.2 μg（r=0.999 9）和1.00-15.0 μg（r=0.999 8），平均加样回收率分别为97.23%，96.27%，RSD分别为1.21%，1.66%。结论：该方法简便，精密度、重复性良好，结果准确可靠，可以作为向日葵列当及其相关制剂的质量控制。

Objective： To establish a RP-HPLC method for simultaneous determination of phenylethanoid glycosides crenatoside and acteoside in Orobanche cumana. Method： The analysis was performed on a Agilent ZORBAX SB-C18 column （4.6 mm250 mm,5 μm） with CH3CN-0.01%HOAc（18：82） as mobile phase at a flow rate of 1.0 mL·min^-1and at a column temperature of 30 ℃,330 nm as the detection wavelength,the well separation was achieved in 30 min. Result： Crenatoside showed good relationship at the range of 1.08-16.2 μg（r=0.999 9）,and acteoside at the range of 1.00-15.0 μg （r=0.999 8）. The average recovery were 97.23% and 96.27%,relative standard derivation （RSD） of 1.21% and 1.66% for crenatoside and acteoside. Conclusion： The proposed method is simple,rapid,accurate and reliable.It can be used for the quality evaluation of O. cumana and its preparation.