芪苈强心胶囊对改善慢性心力衰竭大鼠心功能及AQP-2表达的影响

Effect of Qili Qiangxin Capsule on AQP-2 and Heart Function in Experimentally Induced Heart Failure

目的:探讨芪苈强心胶囊对改善慢性心衰(CHF)大鼠的心功能及水通道蛋白-2(AQP-2)表达的影响及其改善慢性心力衰竭水代谢紊乱的机制。方法:结扎冠脉左前降支,建立急性心肌梗死(AMI)模型,存活者随机分为模型组(等体积水),芪苈强心组(1 g·kg-1·d-1),缬沙坦组(10 mg·kg-1·d-1),另设假手术组(等体积水)。给药4周后,应用超声心动图检测大鼠的心功能;并通过免疫组化和Western blotting检测大鼠肾集合管AQP-2表达。结果:与假手术组相比,模型组左心室舒张末期内径(LVEDD)和左心室舒张末期内径(LVESD)明显增加,射血分数(EF)和短轴缩短分数(FS)显著降低(P<0.05),证实造模成功;与模型组相比,芪苈强心组和缬沙坦组LVEDD和LVESD均明显减小,EF和FS均显著升高(P<0.05);芪苈强心组与缬沙坦组比较,差异无统计学意义;与假手术组相比,模型组AQP-2表达水平显著升高(P<0.05);与模型组相比,芪苈强心和缬沙坦组AQP-2表达均显著降低(P<0.05),两组AQP-2表达比较,差异亦无统计学意义。结论:芪苈强心可有效下调AQP-2的表达水平,调节由慢性心衰导致的水代谢紊乱,改善水肿症状、从而提高慢性心衰患者的生存质量。

Objective： To study Qili Qiangxin capsule（QL）＇ s function in improves the chronic heart failure rats＇ cardiac function and its＇ influence in the aquaporin-2 Water channel expression, then discuss benefit of how Qili Qiangxin therapies for disorders that chronic heart failure rats＇ aberrant water movement. Method： The proximal left anterior descending branch （LAD） of coronary artery was ligated using a terylene suture in acute myocardial infarction（AMI） rats, after building AMI model successfully , survivors were randomly divided into four groups, model group （equal volume of water）, QL group （1g·kg^-1·d-1）, valsartan group （10 mg·kg^-1·d^-1）, sham-operated group （equal volume of water） .Drug intervention four weeks ,then detection of related indicators. Echocardiography and the expressions of aquaporin-2 were detected by the method of immunohistochemistry and western blotting were assessed 4 weeks after the drug therapy. Result： Compared with the sham-operated group, the left ventricular end-diastolic dimension（LVEDD） and left ventricular end-systolic dimension（LVESD） of model group is significantly increased, ejection fractions（EF） and factional shortening（FS） was significantly lower （P〈0.05）; compared with model group, QL group LVEDD and LVESD is significantly reduced , EF and FS were significantly higher （P〈0.05）. There was no significant difference between QL group and valsartan group; compared with the sham-operated group , the aquaporin-2 water channel expression levels were significantly higher in model group（P〈0.05）; compared with the model group, the AQP-2 expression were reduced to varying degrees in QL and valsartan group （P〈0.05）; while there was no significant difference between QL group and valsartan group. Conclusion： QL can be effectively reduced AQP-2 expression levels, improve chronic heart failure caused by the water metabolism, elimination of edema symptoms, thereby improving the quality of life in patients with chronic heart failure.