RP-HPLC法测定葛藤分散片中葛根素与大豆苷的含量

Determination of puerarin and daidzin in Kudzu Dispersible Tablet by RP- HPLC

目的 建立同时测定葛藤分散片中葛根素与大豆苷含量的反相高效液相色谱法.方法 采用Agilent Zarbax Eclipse C18色谱柱（4.6 mm×150 mm,5μm）,流动相为甲醇-水（20：80,V/V）,流速1.0 mL·min-1,检测波长250 nm,柱温35℃,进样量10 μL.结果 葛根素和大豆苷在各自的线性范围内（葛根素：9.04 ～ 72.32 μg·mL-1；大豆苷：9.12 ～72.96 μg·mL-1）,峰面积与浓度呈良好的线性关系（r值均大于0.999）.葛根素和大豆苷的最低定量限分别为0.45和0.46 μg·mL-1,加样回收率分别为100.2％和101.7％,RSD分别为0.7％和0.9％（n=9）.结论 本方法简便、准确、灵敏、重现性好,专属性强,可用于葛藤分散片中葛根素和大豆苷的质量控制.

Objective To establish a RP - HPLC lnethod for the determination of puerarin and daidzin in Kudzu Dis- persible Tablet Methods Agilent Zarbax Eclipse C18 column（4.6mm × 150 mm,5 μm） was adopted with the lnobile phase consisted of methanol - water（20：80, V/V） ,the flow rate was 1.0 mL· min ^-1 and the detection wavelength was set at 250 nln The colulnn temperature was 35 ℃ and the injection volulne was 10 μL Results The linear range of puerarin 9.04 ～72.32 μg ·mL ^-1 and 9.12 ～72.96μ g ·mL ^-1 for daidzin The lower limit of quantification of puerarin and daidzin were 0.45 and 0.46 μg ·mL ^-1 ,respectively The average recovery were 100.2% and 101.7% （ n=9） ,respectively;RSD were 0.7% and 0.9% , respectively Conclusion The lnethod was silnple, specific, sensitive and accurate with good re- peatability,which may be used for the quality control of puerarin and daidzin in Kudzu Dispersible Tablet.