sCD40L在大鼠梗阻性黄疸肝损伤中的作用

Effects of soluble CD40L on hepatic injury of rats with obstructive jaundice

目的探讨可溶性CD40L(soluble CD40L,sCD40L)在大鼠梗阻性黄疸肝损伤中的作用及其与肝损伤严重程度的相关性。方法雄性SD大鼠18只,随机分为模型组、抗CD40L单克隆抗体(antiCD40LmAb)组和假手术组各6只。模型组开腹后游离出胆总管双重结扎即关腹;单抗组同样方法造模后给予腹腔注射anti-CD40LmAb;假手术组仅予游离出胆总管即关腹。分别检测3组大鼠术后不同时间的血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆红素(TBIL)、结合胆红素(DBIL)水平,以及肿瘤坏死因子-α(TNF-α)、白细胞介素-8(IL-8)、sCD40L的浓度。结果与模型组相比,单抗组血清ALT、AST、TNF-α、IL-8及sCD40L显著减低(P<0.05),而TBIL及DBIL水平差异无统计学意义(P>0.05)。血清sCD40L与TNF-α、IL-8的水平呈正相关。结论 sCD40L可能参与梗阻性黄疸免疫肝损伤的启动阶段,阻断CD40/CD40L途径可能成为肝损伤治疗的有效方法之一,sCD40有望成为肝损伤程度的新指标。

Objective This paper is to study the effect of soluble CD40L （sCD40L） on hepatic injury of rat with obstructive jaundice and its correlation with severity of liver damage. Methods Eighteen male SD rats were evenly and randomly divided into model of obstructive jaundice group, treatment of anti CD40L monoclonal antibody （anti CD40LmAb） group and control group. The rats of the model group were cut open the abdomen, dissociated the bile duct and double ligation, and closed the abdomen. After treating with the same method, the anti CD40LmAb group was given anti CD40LmAb intraperitoneal injection. The control group was only dissociated the bile duct and closed the abdomen. Three groups were detected of the serum levels of ALT, AST, TBIL, DBIL and TNF-α, IL-8, and sCD40L at different times. Results Compared with the model group, the ALT, AST, TNF-α, IL-8 and sCD40L levels of the anti CD40LmAb group were significantly lower （P〈0.05）, but the TBIL and DBIL levels had no significant difference （P〉0.05）. The serum sCD40L was positively correlated to those of TNF-α and IL -8. Conclusions sCD40L probably participate in the startup phase of hepatic injury of obstructive jaundice immune. Blocking the CD40/CD40L passway may be one of the effective methods for treatment of hepatic injury. sCD40 is expected to become a new indicator for hepatic damage degree.