HPLC法测定麻杏止咳糖浆中苦杏仁苷的含量

Determination of Amygdalin in Maxing Cough Syrup by HPLC

建立高效液相色谱法测定麻杏止咳糖浆中苦杏仁苷含量的方法。采用KromasilC18柱，乙腈-0.1%磷酸溶液（8∶92）为流动相，流速1.0mL·min-1，检测波长207nm。苦杏仁苷在0.0406～0.812μg范围内有良好的线性关系， r=0.9999，平均回收率为99.0%，RSD为0.25%。本方法简便、准确、重现性好，可用于麻杏止咳糖浆的质量控制。

A HPLC method for determination of amygdalin in Maxing cougy syrup by HPLC was established.Applied KromasilC18 column（250mm×4.6mm,5μm）, the mobile phase was acetonitrile-0.1% phosphoric acid solution（8：92）, the flow rate of 1.0mL·min-1, the detecting wavelength was at 207nm. The linear range of amygdalin was 0.0406~0.8112μg （r=0.9999）,the average recovery was 99.0%, RSD was 0.25%.The method was simple,accurate and reproducible,it could be used for quality control of maxing cough syrup.