亚砷酸联合电离辐射作用于肝癌细胞株HepG2的实验观察

Experimental observation of arsenic trioxide in combination with ionizing radiation on hepatocellular carcinoma HepG2 cells

目的:探讨亚砷酸对肝癌细胞株HepG2的放疗增敏作用。方法:采用细胞培养及传代方法,加入不同浓度亚砷酸,48h后用四唑盐实验(MTT)法检测肝癌细胞株HepG2的抑制率。不同剂量6兆伏X射线照射肝癌细胞株,48h后用平板克隆形成实验观察存活分数。结果:在一定浓度范围内(1.25-10mg/ml),亚砷酸对HepG2细胞增殖的抑制呈浓度依赖性;亚砷酸联合放疗能降低HepG2细胞存活分数,可以使细胞存活曲线的D0值从单纯放疗组的1.94降至1.75,放射增敏比为1.38。结论:亚砷酸对肝癌细胞株HepG2具有放疗增敏作用。

Objective：To investigate the radiotherapy of arsenic trioxide on hepatocellular carcinoma HepG2 cells. Methods：Used the method of cell culture and passage, different concentrations of arsenic trioxide were added in cells. And four tetrazolimn test （MTT） was used to detect the inhibition rote of hepatocellular carcinoma HepG2 cells after 48 hours. Flat cloning experiment was used to observe the survival fi-action of the HepG2 cells after 48 hours by different dose of 6 MeV X - rays. Results：Within a certain concentration range （ 1.25 - 10mg/ml）, arsenic trioxide inhibited the proliferation of HepG2 cells in a concentration dependent manner. Arsenic trioxide in combination with i- onizing radiation can decrease HepG2 cell survival fraction. The D0 value of cell survival curves droped from 1.94 to 1.75 in simple radiotherapy group. The radiosensitization ratio is 1.38. Conclusion：Arsenic trioxide has radiosensiti- zation effect on hepstocellular carcinoma HepG2 cells.