硅钼蓝光度法测定药物中硫普罗宁含量

Spectrophotometric Determination of Tiopronin in Pharmaceutical Sample by Silicomolybdenum Blue

建立了硅钼蓝光度法测定硫普罗宁的新方法，详细探讨了硅钼蓝光度法测定硫普罗宁的各种影响因素。结果表明，在一定的反应条件下，体系中生成的硅钼黄可被硫普罗宁分子中的巯基（-SH）定量还原为硅钼蓝，通过测定硅钼蓝的吸光度，从而间接测定硫普罗宁的含量。显色体系最大吸收波长为686nm，硫普罗宁质量浓度在8．00～80．0tLg／mL范围内与A呈良好的线性关系，线性回归方程为A=-0．0163＋0．0104p（μg／mL），线性相关系数r=0．9995。方法用于测定硫普罗宁药片中硫普罗宁的含量，结果与标准方法相符。

A novel method for spectrophotometric determination of tiopronin by silicomolybdenum blue was established. The effect of various factors on spectrophotometrie determination of tiopronin by silicomolyb- denum blue was investigated in detail. The results showed that under certain reaction conditions silicomo- lybdenum yellow could be reduced to silicomolybdenum blue by the hydrosulfuryl group （-SH） in tiopronin molecule, and the content of tiopronin was determined indirectly through determining the absorbance of silicomolybdenum blue. The maximum absorption wavelength of chromogenic system is 686 nm, and a good linear relationship is obtained between the absorbance and the concentration of tiopronin in the range of 8.00-80.0μg/mL. The regress coefficient of 0. 9995. This propose and the results agree well with the ion equation is A=-0. 0163＋0. d method has been applied to d standard method.