来氟米特对脂多糖诱导下HaCaT细胞增殖和凋亡的影响

Effects of leflunomide on the proliferation and apoptosis of the lipopolysaccharide-induced HaCaT cells

目的观察来氟米特活性代谢产物A771726对脂多糖诱导下HaCaT细胞增殖和凋亡的影响。方法采用MTT法检测细胞增殖水平的变化;流式细胞仪检测细胞凋亡;倒置相差显微镜下观察细胞形态。结果 A771726对脂多糖诱导下HaCaT细胞的增殖有明显的抑制作用,具有时间-浓度依赖性;A771726能促进细胞凋亡,凋亡率随浓度的增加而增加;倒置显微镜下观察,脂多糖组细胞呈致密单层,随着A771726浓度升高,细胞体积变小、细胞间隙变大、细胞内空泡增多。结论来氟米特可以抑制脂多糖诱导下HaCaT细胞增殖,促进其凋亡,这可能是来氟米特治疗银屑病的作用机制之一。

Objective To observe the proliferation and apoptosis of the lipopolysaccharide （LPS）-induced HaCaT cells in the presence of the active leflunomide metabolite A 771726 .Methods MTT was used to detect the level of cell proliferation;flow cytometry （ FCM） was used to detect apoptosis of HaCaT cells;inverted phase contrast＆nbsp;microscope （IPCM） was used to observe morphologic changes .Results The A771726 significantly inhibited the pro-liferation of LPS-induced HaCaT cells , the inhibition effect was concentration-and-time dependent;the A771726 pro-moted cell apoptosis and the apoptosis rate increased with its concentration , the LPS group showed dense monolayer cells under an inverted microscope , as the concentration of the A 771726 increased , the volume of the cells got smal-ler and intercellular space got larger , and the more vacuoles was observed in the cells .Conclusion Leflunomide can inhibit the proliferation of the LPS induced HaCaT cells and expedite their apoptosis , which may be one of the mecha-nisms of leflunomide in the treatment of psoriasis .