明胶/聚己内酯纳米纤维电纺膜在表皮组织工程中的应用

Electrospun Nanofibrous Gelatin/Polycaprolactone Membrane for Epidermis Engineering

目的观察明胶/聚己内酯(GT/PCL)纳米纤维电纺膜应用于表皮组织工程的可行性。方法测定HaCaT细胞(人类表皮细胞系)与电纺膜的生物相容性,并对于接种细胞前后该膜片的机械性能进行评价。应用CCK-8法检测细胞增殖情况。体内研究检测GT/PCL纳米纤维电纺膜构建的组织工程化表皮修复裸鼠皮肤缺损的效果。结果 HaCaT细胞能够在膜片上很好地附着和增殖,该膜片具有良好的生物相容性。机械性能测试显示,该膜片具有足够的力学特性以用于移植。体内研究表明,应用GT/PCL纳米纤维电纺膜构建的组织工程化表皮能够修复裸鼠的皮肤缺损。结论 GT/PCL纳米纤维电纺膜是一种适合构建组织工程化表皮的支架材料。

Objective To investigate the feasibility of a modified gelatin and polycaprolactone （GT/PCL） electrospun nanofibrous membrane for epidermis engineering. Methods The biocompatibility of the membrane was evaluated by seeding HaCaT cells （human keratinocyte cell line） on the membrane and the mechanical properties of the membrane were determined before and after seeding HaCaT cells. Cell proliferation assay was tested by CCK-8 kit. Then the outcome of GT/PCL membranes repairing skin defects in the nude mouse was investigated. Results The cell proliferation assay showed good attachment and proliferation of HaCaT cells on the membranes and demonstrated good biocompatibility of the membranes. The membranes were strong enough to be transplanted confirming by mechanical test. Further in vivo transplantation studies revealed that engineered epidermis with GT/PCL membranes are able to repair skin defects of the nude mouse. Conclusion The modified GT/PCL electronspun nanofibrous membrane is a kind of suitable scaffold for epidermis engineering.