摘要
高质量的基因组DNA提取是进行广西莪术生物技术研究的重要基础．本文采用改良CTAB法、改良SDS法和试剂盒分别提取广西莪术叶片的基因组DNA，并进行电泳和核酸蛋白含量检测以及PCR验证．结果表明改良CTAB法提取广西莪术基因组DNA样品的纯度高，杂质少，而且能有效的进行PCR扩增．说明改良CTAB法是一种高效的提取广西莪术DNA的方法．
AIM： The extraction of high quality genomie DNA is an important foundation for the bioteehnology research of eurcu- ma. METHODS： This research used the modified CTAB meth- od, SDS method and reagent kits respeetively, to extract leaves of genomie DNA from Guangxi Cureuma. The quality were validated by DNA eleetrophoresis, nucleic acid protein content detection and PCR. RESULTS： The results show that the modified CTAB method can obtain high purity, less impurity genomic DNA, and PCR amplification. CONCLUSION： The modified CTAB method is a highly effective method for extracting DNA from Cureuma.
出处
《转化医学电子杂志》
2014年第2期69-71,共3页
E-Journal of Translational Medicine
基金
广西教育厅科研项目（200810MS143）
