Wnt5a在胃癌的表达及其临床意义

Expression and clinical significance of Wnt5a in gastric carcinoma

目的:观察Wnt5a在胃癌中的表达及意义。方法:采用RT-PCR法检测Wnt5a基因在正常胃黏膜细胞GES-1及胃腺癌细胞SGC7901中的表达;应用Western-blot法测定GES-1细胞及SGC7901细胞Wnt5a蛋白的表达;运用免疫组化SP法观察60例胃腺癌及30例癌旁胃黏膜组织Wnt5a蛋白的表达,分析Wnt5a表达与胃癌临床病理参数间的关系。结果:Wnt5a基因与蛋白在SGC7901细胞中的表达高于GES-1细胞;Wnt5a在胃癌组织中的表达高于癌旁正常胃黏膜组织(P<0.05)。淋巴结转移及TNM分期越晚的胃癌组织Wnt5a表达越高(P<0.05)。结论:Wnt5a与胃癌的侵袭和转移有关,在胃癌的发生发展中起癌基因样作用。

Objective To study the significance of Wnt5a gene expression in gastric carcinoma （GC）. Methods The Wnt5a mRNA expressions in SGC7901 cell line and normal gastric mucosa GES-1 cell line were detected by RT-PCR. The Wnt5a protein expressions in 60 GC and 30 matched tumor-adjacent normal tissue samples were examined by immunohistochemistry using the streptavidin-peroxidase method. X2 test was used to analyze Wnt5a expression on the clinical pathologic features of gastric carcinoma. Results Both Wnt5a mRNA and Wnt5a protein were significantly higher in SGC7901 cells than those in GES-1 cells. The positive rate of Wnt5a protein expression was also significantly higher in GC than that in tumor-adjacent normal tissue （P 〈 0.05）. Positive expression of Wnt5a was associated with higher degree of regional lymph node metastasis and the later TNM stage （P 〈 0.05）. Conclusion Higher expression of Wnt5a seems to promote invasion and metastasis of gastric cancer, and Wnt5a might play an oneogene-like role in the development of gastric carcinoma.