高危型人乳头状瘤病毒阳性患者支原体属感染与宫颈癌发生的相关性研究

Correlation between Mycoplasma infection and cervical cancer in high-risk HPV-postive patients

目的研究支原体属及高危型人乳头状瘤病毒(HR-HPV)感染与宫颈癌之间是否存在相关性,为临床诊治提供依据。方法选取2011年10月-2012年7月232例宫颈癌患者作为宫颈癌组,256例宫颈上皮内瘤样病变(CIN)患者作为CIN组,同时选择240例同期年龄分布频率匹配、健康、宫颈光滑的体检妇女作为对照组;用聚合酶链反应(PCR)+膜杂交法对宫颈癌组、CIN组、对照组进行HR-HPV感染的检测;用PCR-荧光探针法检测HRHPV阳性的宫颈癌组、CIN组、对照组中的解脲脲支原体(Uu)、细小脲原体(Up)的感染情况。结果在HR-HPV阳性的情况下,Uu、Up在宫颈癌组、CIN组、对照组中的感染率分别为70.69%、41.38%;78.12%、43.75%;41.67%、16.67%,与对照组相比差异有统计学意义(P<0.05);Uu、Up>1.0×106 cps/g DNA负荷量在宫颈癌组中、CIN组、对照组中的分布为65.85%、68.00%、24.00%;68.00%、76.00%、8.00%,与对照组相比差异有统计学意义(P<0.05)。结论在HR-HPV阳性的情况下,Uu、Up可能与宫颈癌的发生相关。

OBJECTIVE To study the correlation between Mycoplasma and high-risk human papillomavirus（HRHPV）infection and cervical cancer so as to provide a basis for clinical diagnosis and treatment.METHODS A total of 232cases of cervical cancer patients and a total of 256cases of cervical intraepithelial neoplasia（CIN）patients from Oct 2011to Jul 2012were respectively divided into cervical cancer group and CIN group;meanwhile,240cases of healthy females who received physical examination at the same time with matched age distribution and smooth cervix were chosen into control group;polymerase chain reaction（PCR）＋ membrane hybridization method was adopted to test HR-HPV infection for cervical cancer group,CIN group and control group;PCR-fluorescence probe was adopted for detecting the infection profile of Mycoplasma hominis（Uu）and Urealpasma parvum in HR-HPV positive cervical cancer group,CIN group and control group.RESULTS In the case of the positive HR-HPV,the infection rates of Uu and Up in cervical cancer group,CIN group and the control group were： 70.69%and 41.38%,78.12% and 43.75% as well as 41.67% and 16.67% respectively.Comparing with the control group,there were significant differences（P〈0.05）.Uu and Up with DNA load more than 1.0×106 cps/ g accounted for 65.85%and 68.00%,24.00%and 68.00%,and 76.00%and 8.00%respectively in the acervical cancer group,CIN group and the control group;there was significant differences（P〈0.05）.CONCLUSIONIn the case of the positive HR-HPV,cervical cancer may have correlation with Uu and Up.