Cullin7蛋白在乳腺浸润性导管癌中的表达及临床意义

Expression of Cullin7 in breast invasive ductal carcinoma and its clinical significance

目的探讨Cullin7蛋白在乳腺浸润性导管癌表达及其与临床病理因素之间的关系。方法应用免疫组织化学链霉菌抗生物素蛋白一过氧化物酶（sP）法检测13例正常乳腺组织、20例良性乳腺肿瘤、68例乳腺癌和32例乳腺癌腋窝淋巴结转移灶组织标本中Cullin7蛋白的表达。结果正常乳腺组织组、良性乳腺肿瘤组、乳腺浸润性导管癌组及乳腺癌腋窝淋巴结转移灶组中Cullin7蛋白强阳性表达率分别是0、30．0％、55．9％、78．1％。乳腺癌腋窝淋巴结转移灶组Cullin7蛋白强阳性表达率明显高于其他3组（P〈0．05）。Cullin7蛋白表达与是否绝经、肿瘤大小、临床分期、病理类型之间无相关（P〉0．05），但与组织分化程度、腋窝淋巴结是否转移之间有明显相关（P〈0．05）。Cullin7蛋白在乳腺癌组织中的表达分别与雌激素受体（ER）呈负相关（P〈0．05），但与孕激素受体（PR）、人类表皮生长因子受体2（Her-2）无相关（P〉0．05）。结论Cullin7可能在乳腺癌的发生发展以及侵袭转移中发挥重要的促进作用。

Objective To explore the expression of Cullin7 protein in breast invasive duetal carcinoma and its relationship with the elinieopathologieal factors. Methods The expression of Cullin7 protein was detected in 13 eases of normal breast tissues, 20 cases of benign breast tumor, 68 eases of breast cancer and 32 eases of axillary lymph node metastasis of breast cancer tissue samples by immunohistoehemieal streptavid-in-peroxidase （SP） method. Results The positive expression rate of Cullin7 in normal breast tissue, benign breast tumor, breast invasive duetal carcinoma and axillary lymph node metastases of breast cancer was 0, 30. 0% , 55.9% , and 78. 1% respectively. The positive expression rate of Cullin7 in the axillary lymph node metastases of breast cancer was significantly higher than the other three kinds of tissue samples （P 〈 0. 05 ）. Cullin7 protein expression had no obvious correlation with the menopausal status, tumor size, clinical stage or pathological type P 〉 0. 05 ）, but had a significant correlation with tissue dif- ferentiation degree and axillary lymph node metastasis （P 〈 0. 05 ）. Cullin7 protein expression in breast cancer tissue was significantly negatively correlated with estrogen receptor （ ER, P 〈 0. 05 ）, but not with progesterone receptor （PR） , and human epidermalgrowth factor receptor-2 （ Her-2 ,P 〉 0. 05 ）. Conclusion Cullin7 may play an important role in the development, invasion and metastasis of breast cancer.