他莫昔芬对雌激素受体阴性乳腺癌细胞杀伤能力的研究

Killing capability of tamoxifen to estrogen receptor negative breast cancer cells in vitro

目的观察他莫昔芬（TAM）联合MCF-7细胞株上清对MDB—DA231细胞株存活率的影响。方法细胞计数试剂盒（CCK-8）检测TAM对MCF-7和MDA—MB-231杀伤能力的差异；CCK-8及流式细胞仪（FCM）检测MCF-7细胞株上清对MDB-DA231细胞株存活率的影响以及MCF-7细胞株上清联合TAM对MDB-DA231细胞株存活率的影响。结果在15μmol／LTAM培养条件下，MCF-7细胞存活率低于MDA—MB-231细胞存活率[（3．03±0．32）％比（45．06±14．29）％]，差异有统计学意义（P〈0．05）；在MCF-7细胞上清培养条件下，MDA-MB-231细胞存活率低于培养基对照组[（83．79±2．58）％比（99．99±1．07）％）]，差异有统计学意义（P〈0．05）；在MCF-7细胞株上清联合TAM培养条件下，MDA—MB-231细胞存活率低于培养基对照组[（1．61±0．15）％比（99．99±1．07）％]，差异有统计学意义（P〈0．05）。在15μmol／LTAM培养条件下，MDA—MB-231被抑制在G0～G1期[（59．14±0．39）％比（50．28±0．0．52）％]，差异有统计学意义（P〈0．05）；在MCF-7细胞上清培养条件下，MDA—MB-231细胞周期无明显变化（P〉0．05）；在MCF-7细胞株上清联合TAM培养条件下，MDA—MB-231被抑制在G0-G1期[（60．79±0．67）％比（59．14±0．39）％]，差异有统计学意义（P〈0．05）。结论MCF-7细胞上清能够增强TAM对MDA—MB-231细胞株的杀伤能力。

Objective To study the effect of tamoxifen combined with the supematant of MCF-7 cell lines on the survival of MDB-DA231 cell lines in vitro. Methods Cell counting kit-8 （CCK-8） was used to analyze the difference of killing by tamoxifen between 1.0 × 10^5/pore MDA-MB-231 and MCF-7. CCK-8 and flow eytometry （FCM） were used to analyze the killing of the supematant of MCF-7 cell lines to MDB-DA231 cell lines and the killing of tamoxifen combined with the supematant of MCF-7 cell lines to 1.0 × 10^5/pore MDB-DA231 cell lines. Results The survival rate of MCF-7 was lower than that of MDA-MB-231 cultured with 15 μmol/L of tamoxifen [ （3.03 ± 0. 32）% vs. （45.06 ± 14. 29 ）% ,P 〈 0. 05 ]. After culture with the supematant of MCF-7 cells, the survival rate of MDA-MB-231 cells was increased [ （ 83.79 ± 2. 58 ） % vs. （99. 99 ± 1.07 ） %, P 〈 0. 05 ]. After culture with tamoxifen combined with the supernatant of MCF-7 cells, the survival rate of MDA-MB-231 cells was significantly increased [ （ 1.61 ± 0. 15）% vs. （99. 99 ± 1.07）% ,P 〈0. 05]. After culture with 15 μmol/L of tamoxifen, the cell cycle of MDA-MB-231 cells was arrested in G0-G1 phase [ （ 59. 14 ± 0. 39 ） % vs. （50. 28 ± 0. 0. 52 ） %, P 〈 0. 05 ]. After culture with the supematant of MCF-7 cells, no eel1 cycle arrest was found in MDA-MB-231 cells （P 〉 0. 05 ）. After culture of tamoxifen combined with the supematant of MCF-7 cells, the eel1 cycle of MDA-MB-231 cells was arrested in GO-G1 phase [ （ 60. 79 ± 0. 67 ） % vs. （ 59. 14 ± 0. 39 ） %, P 〈 0. 05 ]. Conclusion The supernatant of MCF-7 cells could enhance the killing ability of tamoxifen to MDA-MB-231 cells.