摘要
目的观察二氧化铈（CeO2）纳米颗粒对大鼠心肌缺血再灌注损伤的保护作用。方法将SD大鼠50只随机分为假手术组、模型组、1～10nm粒径组、10～25nm粒径组、50nm粒径组，每组10只，制作缺血再灌注模型。采用黄嘌呤氧化酶法和比色法分别测定超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH—Px）活性，硫代巴比妥酸法测定丙二醛（MDA）含量，同时观察心肌组织病理学和细胞凋亡。结果与假手术组比较，模型组SOD、GSH—Px活性明显下降，MDA含量明显升高（P〈0．05）；与模型组比较，3种不同粒径组SOD、GSH—Px活性明显升高，MDA含量明显降低（P〈0．05）。3种不同粒径组心肌细胞凋亡指数[1—10nm组：（21．00±3．90）％；10～25nm组：（16．38±3．59）％；50nm组：（24．20±5．88）％]与模型组[（32．92±7．46）％]比较明显降低（P〈0．05）。结论二氧化铈纳米颗粒能减轻大鼠缺血再灌注心肌的氧化应激，具有较好的心肌保护作用。
Objective To investigate the protective effects of cerium oxide （CeO2 ） nanoparticles on myocardial ischemia-repeffusion injury in rats. Methods Fifty male Sprague-Dawley rats were randomly divided into five groups： sham group, experimental group and three preconditioning groups with different particle diameter [ 1-10 nm group, 10-25 nm group and 50 nm group （ n = 10 in each group） 1- The myocardial ischemia-repeffusion model was established successfully. The activities of superoxide dismutase （SOD） and glutathione （GSH） -Px, and the content of malondialdehyde （MDA） in myocardial tissue were determined. The morphology of myocardium was observed under a light microscope, and the apoptosis of myocardial cells was detected by using TdT-mediated dUTP nick end labeling （TUNEL） method. Results As compared with the Sham group, the activities of SOD and GSH-Px in experimental group were signifi- cantly decreased （ P 〈 0. 05 ） , while the content of MDA was increased （ P 〈 0. 05 ）. The activities of SOD and GSH-Px were increased and the content of MDA was decreased in the three different particle diameter groups as compared with the experimental group （ P 〈 0. 05 ）. The apoptosis of myocardial cells was significantly decreased in the three different particle diameter groups I （21.00 ± 3.90 ）% for 1-10 nm group; （ 16. 38 ± 3.59 ） % for 10-25 nm group ; and （ 24. 20 ± 5.88 ） % for 50 nm group] as compared the experi- mental group [ （32. 92 ± 7.46） % ] （ P 〈 0. 05）. Conclusion The CeO2 nanopartieles can protect the myocardium from isehemia-repeffusion injury by attenuating oxidative stress.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第5期1060-1062,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（81241003）
河南省科技创新杰出人才计划资助项目（104200510007）
郑州市科技创新人才培育计划资助项目（10LJRC175）
