Dicer在甲状腺乳头状癌中的表达及其临床意义

Expression of dicer in papillary thyroid carcinoma and its clinical significance

目的观察Dicer基因在甲状腺乳头状癌（PTC）中的表达，探讨Dicer基因表达与PTC的发生发展的关系。方法采用免疫组织化学链菌素抗生物素蛋白一过氧化物酶（SP）法和荧光定量逆转录聚合酶链反应技术检测Dicer在50例PTC及其配对的癌旁组织（NCE）的蛋白及mRNA的表达。结果DicermRNA在PTC和NCE中的阳性表达率分别为80％（40／50），10％（550），其在PTC中的表达显著高于NCE（P〈0．01）。Dicer蛋白在PTC和NCE中的阳性表达率分别为76％（38／50），8％（4／50），其在PTC中的表达明显高于NCE（P〈0．01）。Dicer蛋白及其mRNA在PTC中的表达与患者的TNM分期及淋巴转移有关（P〈0．05）。Dicer蛋白和DicermRNA在PTC中的表达有明显的相关性（P〈0．01）。结论Dicer在PTC发生发展中起到重要作用。

Objective To investigate the Dicer gene expression and its clinicopathological signifi- cance in papillary thyroid carcinoma, and the relationship between the Dicer gene expression and tumorogenesis of papillary thyroid carcinoma. Methods The immunohistochemical streptavidin avidin-peroxidase （SP） technique and real-time quantitative polymerase chain reaction （ Real-time PCR） were used to detect the expression of Dicer protein and mRNA in 50 cases of PTC and their matched adjacent non-cancerous epithelia （NCE）. Results The positive expression rate of Dicer mRNA in PTC and NCE wase 80% （40/50） and 10% （5/50） respectively （P 〈0. 01 ）. The positive expression rate of Dicer protein in PTC and NCE was 76% （38/50） and 8% （4/50） respectively （P 〈 0. 01 ）. The expression of Dicer protein and mRNA was associated with the invasion and metastasis of PTC （ P 〈 0. 05 ）. The expression of Dicer protein and mRNA showed a distinct correlation （ P 〈 0. 01 ）. Conclusion Dicer plays a pivotal role in the carcinogenesis and progression of PTC. Dicer could be a new diagnostic/therapeutic target in PTC.