AT富集序列特异性结合蛋白1在直肠癌组织中的表达及其临床意义

Expression of special AT-rich sequence-binding protein in rectal cancer and its clinical significance

目的探讨AT富集序列特异性结合蛋白1（SATBI）在直肠癌组织中的表达及其临床意义。方法分别采用实时定量聚合酶链反应（Real—timePCR）和免疫组织化学法检测直肠癌组织中SATB1mRNA与蛋白水平的表达，并分析两者相关性；应用统计学方法分析SATB1表达与患者临床病理参数之间的关系及其预后意义。结果直肠癌及正常对照组织中SATB1mRNA相对表达量分别为1．93±0．36和0．75±0．19，蛋白阳性表达率分别为42．5％和6．25％，直肠癌组织均显著高于正常组织（P〈0．05）；SATB1mRNA与其蛋白表达水平呈显著正相关（r=0．649；P〈0．05）。SATB1高表达与直肠癌患者较高的TNM分期、淋巴结转移及远处转移之间相关（P〈0．05）。结论SATB1在直肠癌组织中的表达水平显著上调；SATB1高表达与直肠癌的侵袭、转移潜能密切相关。

Objective To investigate special AT-rich sequence binding protein 1 （SATB1） expression and its clinical significance in the rectal cancer tissues. Methods The expression of SATB1 mRNA and protein was detected by real-time quantitative polymerase chain reaction （Real-time PCR） and immunohistochemical staining respectively in 80 cases of rectal cancer. The correlations between SATB1 expression and clinicopathological factors and its prognostic significance were statistically analyzed. Results The relative levels of SATB1 mRNA in rectal cancer tissues and normal tissues were 1.93 ± 0. 36 and 0. 75 ±0. 19, respectively. The positive rate of SATB1 protein in cancer and control tissues was 42. 5% and 6. 25% , respectively. Both SATB1 mRNA and protein expression was significantly up-regulated in rectal cancer specimens as compared with that in normal tissues （P 〈0. 05 for both）, and the SATB1 mRNA expression agreed well with the immunohistochemical findings （ r = 0. 649 ; P 〈 0. 05 ）. Moreover, SATB1 expression was positively correlated with higher TNM stage, lymph node metastasis and distant metastasis （P 〈 0. 05 for each）. Conclusion Over-expression of SATB1 could be associated with the developmental and metastatic potential of human rectal cancer.