一株节杆菌发酵产物中类胡萝卜素的分离及组分分析的初探

Preliminary separation and qualitative analysis of carotenoids in Arthrobacter sp.

【目的】对实验室保存的一株红色球菌进行菌株鉴定,并对色素提取液的组分进行定性分析。【方法】采取形态学观察、16S rDNA序列同源性分析和生理生化分析相结合的方法确定菌株的分类地位。使用乙醇-丙酮混合液提取胞内色素,采用硅胶G薄板层析法对色素提取液进行初步分离纯化,并结合其光谱吸收特性、质谱结果进行定性分析。【结果】实验结果表明此菌株属于节杆菌属(Arthrobacter sp.)。薄板层析结果显示该菌株内主要有4种色素组分,且靠近溶剂前沿组分为黄色,其它组分皆为橘红色。吸收光谱特性、质谱结果显示黄色组分可能为β-胡萝卜素,橘红色组分可能为螺菌黄质系类胡萝卜素。【结论】此株节杆菌以较为廉价的糖蜜和玉米浆作为营养物质用于满足自身生长需要,并且积累胞内类胡萝卜素,因此该菌的进一步研究有一定的价值。

[Objective] To analyse the taxonomic status of the red-coccus bacterium conserved in our laboratory, and the compositions of pigments extract were determined. [Methods] This bacterium was classified based on morphological features, 16S rRNA gene sequences and physiological tests. Total pigments were extracted from biomass using ethanol-acetone. Preliminary separation of pig- ments was achieved by thin layer chromatography, and UV/visible scanning spectra and mass spectra were used for the qualitative analysis of each composition. [Results] This bacterium was classified to the genus of Arthrobacter sp.. There were main four compositions on the silica-gel plate. Only one next to the solvent front was yellow, and others were orange. The spectroscopic data and mass spec- tra showed that the yellow and red components might be the β-carotene and spirillloxanthin series respectively. [Conclusion] The production of carotenoids by the strains of Arthrobacter sp. was ob- served, when cheap materials such as molasses and corn steep liquor were used as nutrient medium, and further investigations will have practical values.