一株海洋低温葡萄糖氧化酶菌株的筛选、鉴定及部分酶学性质

A strain of marine low-temperature glucose oxidase screening, identification and the preliminary research of part enzymology properties

【目的】从海洋样品中分离筛选出产葡萄糖氧化酶菌株。【方法】采用双层平板筛选法进行初筛、复筛确定一株酶活较好的菌株,命名为GOD2(Glucose oxidase)。通过形态学、生理生化特征及16S rRNA基因序列分析研究其分类地位,并对其产生的葡萄糖氧化酶进行分离纯化和部分酶学性质的研究。【结果】细菌GOD2为产葡萄糖氧化酶菌株且遗传稳定,初步鉴定该菌株为假单胞杆菌(Pseudomonas migulae),其所产酶最适反应温度为20°C,热稳定性较差,40°C剩余相对酶活80%;超过40°C酶活力迅速下降。【结论】GOD2是一株极具研究价值的产低温葡萄糖氧化酶菌株。目前没有关于利用该菌生产葡萄糖氧化酶的报道。

[Objective] To isolate strains producing glucose oxidase from the sample of sea mud and sea sand in Yellow Sea. [Methods] The strain isolated by plate culture and enyme activity determination, which was identified based on its morphology characteristics, physiobiochemical characteristics and 16S rRNA gene sequence analysis, parts of the glucose oxidase properties were studied, also. [Results] The strain GOD2 （glucose oxidase） was identified as Pseudomonas （Pseudomonas migulae）, and the optimum reaction temperature of the enzyme was 20 ℃, with the poor thermal stability, the relative activity of the remaining 80% at 40 ℃. Enzyme activity decline rapidly above 40 ℃. [Conclusion] GOD2 had a highly research value, and there is no report about it to produce glucose oxidase, at present.