底质农残测定中固相柱净化体系的选择

Study on Clean-Uplethod for Pescide in Sediment Using Solid Phase Traction Column

使用固相柱对底质中的7种农残进行净化。结果表明,在相同淋洗条件下,使用氟罗里硅土柱比硅胶柱净化效果好。比较了不同溶剂体系、不同溶剂配比下2种萃取柱的净化效果,选择乙醚-正己烷(体积比1∶2)或丙酮-正己烷(体积比1∶4)作为净化溶剂,较小的收集体积都可满足所有目标化合物的回收率要求;相比较而言,丙酮-正己烷(体积比1∶4)作为净化溶剂,洗脱液的杂质干扰更小,净化效果更好。使用氟罗里硅土柱和丙酮-正己烷(体积比1∶4)对样品净化,方法简单,快速,净化效果好,适合大批量底质样品的前处理净化过程。

Sample clean-up procedure based on solid-phase extraction （SPE） was developed for the simultaneously determination of 7 organic pesticides in deposition samples. The study shows clean-up using Florisil type SPE column can do better job than silica type SPE column under the same condition. The behaviors ofeluting solvents with different composition were tested and compared. Higher recovery of individual compounds and less volume of elution solvent can be achieved by mixture solvent of acetone-hexane （volume ratio 1：4）. So,it is a simple and rapid method to use Florisil column with elution solvent of acetone- hexane （ volume ratio 1 ： 4）.