T-cadherin在胃癌中的表达及其与VEGF、MVD之间的关系

T-cadherin expression in gastric cancer and its relationship with VEGF and MVD

目的:探讨T-cadherin在胃癌组织中表达及其与VEGF、MVD之间的关系。方法:采用免疫组化SP法对80例胃癌组织及癌旁正常胃组织的T-cadherin、血管内皮生长因子(Vascular endothelial growth factor,VEGF)和CD34的表达情况进行检测。结果:T-cadherin主要在胃癌细胞膜上表达,阳性率为45.0%(36/80),而癌旁正常胃组织中T-cadherin阳性率为62.5%(50/80),两者比较差异有统计学意义(P=0.026)。VEGF在癌旁正常细胞阳性率为28.75%,在胃癌中表达阳性率为71.25%,差异有统计学意义(P=0.000)。CD34阳性细胞着色部位主要是血管内皮细胞的胞膜和胞浆,胃癌中标记的微血管明显多于正常组织(54.96±7.28/HPF vs 27.50±6.10/HPF,P<0.001)。T-cadherin和VEGF蛋白表达呈负相关(P=0.001);T-cadherin阳性组的MVD值明显低于阴性低表达组(P<0.001).T-cadherin、VEGF、CD34 3种蛋白在胃癌中的阳性表达与患者性别和年龄、肿瘤大小均无关(P>0.05),但与组织分化程度、淋巴结转移及pTNM分期显著相关(P<0.05)。结论:T-cadherin的异常表达可以作为临床评价胃癌诊断、指导治疗及判断预后的辅助指标之一。

Objective：To discuss T - cadherin exPression in gastric cancer and the relationshiP between VEGF, MVD. Methods：Using immunohistochemical SP method to test the exPression of T - cadherin,vascular endothelial growth factor（VEGF）and CD34 in 80 cases of gastric cancer tissue and normal gastric tissue adjacent to carcinoma. Results：T - cadherin exPressied in the gastric cancer cell membrane,Positive rate was 45. 0%（36 / 80）,while in normal gastric tissues adjacent to carcinoma T - cadherin Positive rate was 62. 5%（50 / 80,P = 0. 026）. VEGF in normal cells adjacent to carcinoma Positive rate was 28. 75% ,the exPression Positive rate was 71. 25% in cancer of the stomach（P = 0. 000）. CD34 Positive cells mark of caPillaries in gastric cancer significantly more than the normal tissue（54. 96 ± 7. 28 / ePF vs 27. 50 ± 6. 10 / ePF,P ﹤ 0. 001）. T - cadherin was negatively correlated with VEGF Protein exPression（P ﹤ 0. 001）. The MVD value of T - cadherin Positive grouP was obviously lower than negative low exPression grouP（P ﹤ 0. 001）. The T - cadherin,VEGF and CD34 in gastric cancer Patients were not correlatad gender,age,size,（ P ﹥ 0 . 05 ）,but with tissue differentiation degree and lymPh node metastasis and PTNM stage （P ﹤ 0. 05）. Conclusion：T - cadherin abnormal exPression can be used for gastric cancer diagnosis,treatment and Prognosis evaluation.