黄连素对肺腺癌A549细胞增殖、迁移和侵袭的影响

Effects of Berberine on proliferation,migration and invasion of A549 lung adenocarcinoma cells

目的:研究黄连素(BBR)对A549细胞增殖、迁移和侵袭的影响,初步探讨BBR抑制A549细胞转移的机制。方法:用不同浓度的BBR作用于A549细胞,MTT法测定A549细胞增殖水平,流式细胞仪检测A549细胞的凋亡,Transwell实验测定A549细胞的迁移和侵袭能力,Western blot测定A549细胞中与EMT相关的蛋白E-cadherin、Vimentin、Slug、Snail的表达。结果:BBR以浓度及时间依赖的方式抑制A549细胞的增殖;流式细胞仪检测发现BBR作用于A549细胞后凋亡率呈剂量依赖性增加;Western blot显示BBR作用A549细胞后,E-cadherin表达升高,Vimentin、Slug、Snail表达降低,Transwell实验显示BBR作用于A549细胞后,能够抑制其迁移和侵袭。结论:BBR能抑制A549细胞增殖,诱导其凋亡,通过促进E-cadherin表达和抑制Vimentin、Slug、Snail的表达来抑制EMT的发生,从而抑制了A549细胞的迁移和侵袭。

Objective：To study the effect of Berberine on Proliferation,migration and invasion of A549 cells. Methods：The Proliferation and aPoPtosis of A549 cells were determined by MTT and FACS resPectively. The rate of A549 cell migration was observed by Transwell assay. The exPression of E - cadherin,Vimentin,Slug,Snail in A549 cells effected by Berberine were assessed by Western blot. Results：The Proliferation of A549 cell could be significant-ly inhibited within limited duration and dosage（P ﹤ 0. 05）. Also exPression of Vimentin,Snail and Slug of gene were decreased but E - cadherin increased after Berberine treatment（P ﹤ 0. 05）. Conclusion：Berberine can inhibit the mi-gration abilities of A549 cells that might be related to down - regulating the exPression of Vimentin,Snail and uP -regulating the exPression of E - cadherin.