PEDF与VEGF在肝外胆管癌中的表达及意义

Expression and significance of PEDF and VEGF in extra-hepatic cholangiocarcinoma

目的:探讨色素衍生上皮因子(PEDF)及血管内皮生长因子(VEGF)表达与肝外胆管癌的关系,并对胆管癌组织中两者的相关性进行初步研究。方法:收集中国医科大学附属盛京医院普通外科于2006年1月-2010年5月手术切除并经病理证实的肝外胆管癌标本70例。30例正常胆管组织取自胆囊结石患者行胆囊切除且无炎症状态的胆囊管组织。用免疫组织化学方法检测PEDF及VEGF的表达情况,统计学处理采用SPSS 19.0软件,组间关系采用单因素方差分析,相关关系分析采用Spearman等级相关分析法。结果:PEDF表达于细胞膜和细胞浆,以细胞膜和细胞浆出现棕黄色为阳性表达,其在胆管癌组织中有阴性或弱阳性表达,在正常胆管组织中有阳性表达。VEGF表达于细胞浆,以细胞浆出现棕黄色或棕褐色颗粒为阳性表达,其在胆管癌组织中有阳性表达,在正常胆管组织中为阴性或弱阳性表达。在胆管癌组织中PEDF与VEGF的表达呈负相关。PEDF和VEGF在胆管癌中的表达与有无淋巴结转移及肿瘤分期有关,而与肿瘤大小、分化程度无关。结论:PEDF表达减少或VEGF表达增高与胆管癌的发生、发展及侵袭转移有关。PEDF与VEGF在胆管癌的发生和发展中起到相反的作用。

Objective：The PEDF and VEGF exPression in extrahePatic bile duct carcinoma tissue and normal tis-sue were assessed by immunohistochemistry to study the correlation between the exPression of PEDF and VEGF. And we also concern with the relationshiP between their exPression and cholangiocarcinoma. Methods：Collect extrahePatic bile duct carcinoma tissue from 70 cholangiocarcinoma Patients normal tissue from 30 cholecystolithiasis Patients＇ bile duct that no iflammation as the samPles in the study. Patients were all PostoPerative and Pathological confirmed in the surgery dePartment of Shengjing eosPital of CMU from January,2006 to May,2010. The PEDF and VEGF exPressions were assessed by immunohistochemistry,and the data was analyzed by SPSS 19. 0. Results：The Positive signal of PEDF exPression was stained yellow and mainly located in the cell cytoPlasma and on the cell membrane. It mainly exPressed negative or weak Positive in carcinoma tissue and Positive or weak Positive in normal tissue. The Positive signal of VEGF exPression was stained yellow or brown and mainly located in the cell cytoPlasma and on the cell membrane. It mainly exPressed Positive in carcinoma,but negative or weak Positive in normal tissue. There was nega-tive correlation between PEDF and VEGF exPressions（r = - 0. 392,P ﹤ 0. 05）. PEDF and VEGF exPression showed the correlation with lymPh node metastasis and neoPlasm staging,but with no correlation with tumor size or histological grades. Conclusion：Low exPression of PEDF has the correlation with occurrence,develoPment,invasion and metasta-sis of cholangiocarcinoma. eigh exPression of VEGF has the correlation with occurrence,develoPment,invasion and me-tastasis of cholangiocarcinoma.PEDF and VEGF have a converse function in the develoPment of cholangiocarcinoma.