胚胎神经上皮干细胞脑内移植治疗大鼠帕金森病

Transplantation of embryonic neuroepithelial stem cells into the brain of Parkinson disease rats

背景:研究表明胚胎干细胞移植可改善血管性痴呆大鼠的学习记忆功能,增强神经的可塑性,诱导自身定向迁移并分化为成熟神经元。目的:观察胚胎神经上皮干细胞脑内移植治疗帕金森病大鼠及移植细胞的迁徙情况。方法:将绿色荧光蛋白转基因鼠的胚胎神经上皮干细胞分别移植到帕金森病大鼠的黑质、纹状体和侧脑室内,移植后检测移植细胞的存活、迁徙与分化;利用高效液相色谱法检测实验动物脑内多巴胺神经递质的含量;对比实验动物旋转行为的改变,评估胚胎神经上皮干细胞移植对帕金森病大鼠的治疗作用。结果与结论:移植细胞存活良好且分化出了酪氨酸羟化酶阳性细胞,向黑质纹状体环路迁徙趋势明显;脑内多巴胺含量增加,动物旋转行为改善明显。表明移植到帕金森病大鼠脑内的神经上皮干细胞多向黑质纹状体环路迁徙,且可增加脑内多巴胺神经递质的含量治疗帕金森病。

BACKGROUND： Studies have shown that embryonic stem ceil transplantation may improve learning and memory abilities in rats with vascular dementia, enhance neural plasticity, stereotaxically migrate and differentiate into mature neurons. OBJECTIVE： To investigate the improvement of Parkinson disease rats following the neuroepithelial stem cells were transplanted into the brain and the migration of the transplanted cells. METHODS： The neuroepithelial stem cells derived from green fluorescent protein transgenic mice were stereotaxically transplanted into the substantia nigra, striatum and lateral cerebral ventricle of Parkinson disease rats. The survival, migration and differentiation of transplanted neuroepithelial stem cells were examined at different time of transplantation, and the relationship between the change of rotation behavior and content of dopamine were analyzed. Therapeutic effects of embryonic neuroepithelial stem cells transplantation in Parkinson disease rats were assessed. RESULTS AND CONCLUSION： Transplanted cells survived well and were positive for tyrosine hydroxylase. Ceils migrated from the transplanted region to the substantia nigra. The rotational behavior of Parkinson＇s diseases rats recovered greatly and the dopamine content was raised after the transplantation of neuroepithelial stem cells. These findings indicate that the neuroepithelial stem cells would migrate to the substantia nigra after transplanted into the brain of Parkinson disease rats, and Parkinson disease could be treated following the increasing of dopamine content in the striatum.