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miRNA-21调节PTEN表达影响宫颈癌HeLa细胞的生物学行为

MiRNA-21 affect the biological behavior of cervical cancer HeLa cells by regulating the expression of PTEN
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摘要 目的:探讨抑制miRNA-21表达对宫颈癌HeLa细胞中PTEN的表达及细胞增殖、侵袭能力的影响。方法:以脂质体介导anti-miRNA-21(anti-miRNA-21转染组)、anti-miRNA-21-neg(阴性对照组)转染HeLa细胞,同时设空白对照组(未转染组)。应用Real-time PCR技术检测3组细胞中miRNA-21的表达,Western blotting检测3组细胞中PTEN的表达,MTT法检测3组细胞的增殖能力,Transwell实验检测3组细胞的侵袭能力。结果:Anti-miR-21转染组与阴性对照组相比,HeLa细胞中miRNA-21的表达量明显降低[(0.187±0.027)vs(0.861±0.144),P<0.01]。转染anti-miRNA-21 96 h后,HeLa细胞增殖抑制率明显升高[(49.44±1.97)%vs(4.36±0.64)%,P<0.01]。Anti-miR-21转染组与阴性、空白对照相比,Hela细胞的侵袭细胞数明显减少[(29.4±2.1)vs(40.4±2.9)、(41.2±2.6)个,均P<0.01];PTEN蛋白的表达则明显增加[(1766.00±35.56)vs(726.00±5.48)、(729.25±17.73),均P<0.01]。结论:抑制miRNA-21的表达后,宫颈癌HeLa细胞增殖、侵袭能力明显下降,其机制可能与上调PTEN的表达有一定关系。
作者 张花 潘莹 孙力 何全中 尹宝靓
机构地区 新乡医学院第三附属医院妇产科
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2014年第2期207-209,共3页 Chinese Journal of Cancer Biotherapy
基金 新乡医学院研究生科研创新支持计划资助项目(No.YJSCX201124Y)~~
关键词 MIRNA-21 宫颈癌 HELA细胞 PTEN
分类号 R737.33 [医药卫生—肿瘤] R730.59 [医药卫生—肿瘤]
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参考文献17

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中国肿瘤生物治疗杂志
2014年 第2期

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